Reviewed, UniProtKB/Swiss-Prot B6ELD1 (PYRD_ALISL)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	VSAL_I1549

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_00225
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity. HAMAP MF_00225
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000100246
Sites
	Active site	175	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6ELD1-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 694D639C70E2E863	FASTA	336	37,026
	10 20 30 40 50 60 MLYRIARAGI FKLDAEKAHD LAIQNFKRFN GTPLDIFYRQ KLVSKPVEVM GIQFKNPIGL 70 80 90 100 110 120 AAGLEKNGEC IEAFGAMGFG FIEVGTVTPR PQAGNDKPRL FRLIEAEGII NRMGFNNLGV 130 140 150 160 170 180 DNLVENVKKA KYDGVIGINI GKNKDTPIEK GTEDYLICME KVYQYAGYIA INISSPNTPG 190 200 210 220 230 240 LRTLQYGEAL DDLLSQLKEK QKELAEKYGK YVPIALKIAP DLDDNELSQI ADSLMKYKID 250 260 270 280 290 300 GVIATNTTLD RSMVEGMKHA EEMGGLSGRP IQTRSTEVVR RLKELLGDSL PIIGVGGVDS 310 320 330 YVAAKEKMVA GADLVQVYSG FIYKGPNLIR DIVNNI

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM178379 Genomic DNA. Translation: CAQ79234.1.
RefSeq	YP_002262981.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6988588.
GenomeReviews	Gene locus VSAL_I1549 in contig FM178379_GR.
KEGG	vsa:VSAL_I1549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351027.
OMA	NAEQQGG.
Family and domain databases
HAMAP	MF_00225. DHO_dh_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ALISL

Accession

Primary (citable) accession number: B6ELD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)