B6EIY5 (SYM_ALISL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	VSAL_I2226

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Homodimer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm By similarity HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	Methionine--tRNA ligase HAMAP MF_00098		PRO_1000093695
Regions
	Domain	585 – 686	102	tRNA-binding
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00098
	Motif	332 – 336	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	146	1	Zinc By similarity
	Metal binding	149	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Metal binding	162	1	Zinc By similarity
	Binding site	335	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6EIY5 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 67C5D25D7E1DE88A	FASTA	686	77,985
	10 20 30 40 50 60 MATDPRKILV TCALPYANGS IHLGHMLEHI QADIWVRYQR LRGNDINFIC ADDAHGTPIM 70 80 90 100 110 120 LKAQQMGITP EEMIAAVSEE HQKDFAGFDI SFDNYHSTHS DENRELASSI YLELKKNGFI 130 140 150 160 170 180 TSRTISQLFD PEKEMFLPDR FVKGTCPKCK SEEQYGDNCD NCGETYSPTD LINPKSAVSG 190 200 210 220 230 240 ATPVMKDSEH FFFDLPQFES MLKEWTRSGS LQTETANKMQ EWFESGLQQW DISRDAPYFG 250 260 270 280 290 300 FEIPGETNKF FYVWLDAPIG YMGSFKNLCD KRDDLDFDEY WKLNSTTELY HFIGKDIVYF 310 320 330 340 350 360 HSLFWPAMLD GAGFRKPNNV FVHGYVTVNG AKMSKSKGTF IKAGTYLNHL DPECLRYYYA 370 380 390 400 410 420 AKLNSRIDDI DLNLEDFTQR VNSDVVNKIV NLASRNAGFI TKRFDGKLSD NFVAPELYNE 430 440 450 460 470 480 FVAASDRIAE LYETREFSRA IREITALADK ANQYIDEKAP WVLAKEEGKE QELQEVASVG 490 500 510 520 530 540 INLFRVLMAY LKPVMPELAA RTEAFLNETL TWECIAQPLT SHEITKFKAL FNRIDPKKVE 550 560 570 580 590 600 AMVEESKEDA AIEMAAKEKA EAEKEKANQT ELDKEPIADE IEFDAFEAVD MRIARIISCE 610 620 630 640 650 660 EIPKANKLLK FQLDIGGETR QVFSGIKSAY TPEELVGKHT VMVANLKPRK MKFGMSEGMI 670 680 LAAGPGGKDL WILEPHEGAQ PGMRVM

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LFI1238.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM178379 Genomic DNA. Translation: CAQ79910.1.
RefSeq	YP_002263591.1. NC_011312.1.
3D structure databases
ProteinModelPortal	B6EIY5.
ModBase	Search...
Protein-protein interaction databases
STRING	B6EIY5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6989051.
GenomeReviews	Gene locus VSAL_I2226 in contig FM178379_GR.
KEGG	vsa:VSAL_I2226.
PATRIC	20854843. VBIAliSal95923_2426.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG721667.
OMA	EEKTRNV.
ProtClustDB	PRK00133.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR014758. Met-tRNA_synth. IPR023458. Met-tRNA_synth_1. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit. TIGR00399. MetG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_ALISL

Accession

Primary (citable) accession number: B6EIY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program