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B6EIN2 (SYL_ALISL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:VSAL_I0990
OrganismAliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]
Taxonomic identifier316275 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 858858Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091284

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B6EIN2 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 68D7F98F624B670D

FASTA85896,816
        10         20         30         40         50         60 
MQEQYNPQDL EQKIQKHWDD SKTFVVTEDA SKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVSRYQRLQG KNVMQPIGWD AFGLPAENAA VKNKTAPAPW TYENIEYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WSREFATCTP EYYRWEQEFF TKLYNKGLVY KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD 

       190        200        210        220        230        240 
TPVEQKKIPQ WFIKITEYAQ ELLDDLDTLD GWPDMVKTMQ RNWIGRSEGV ELSFAVNGEE 

       250        260        270        280        290        300 
APLEVYTTRP DTLMGVTYVG IAAGHPLAEQ AAAKNPELFA FTEECRNTKV AEAELATMEK 

       310        320        330        340        350        360 
KGMDTGLRAI HPLNGREVPI YVANFVLMDY GTGAVMAVPA HDQRDYEFAT KYGLDIIPVI 

       370        380        390        400        410        420 
KPEDGSDVDV SDVAYTEKGV LFDSGEFDGL AFQDAFDAIA AKLEAEGKGK KTVNFRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMVTTEDG EVHPVPADQL PVILPEDVVM DGVTSPIKAD KEWAKTTFNG 

       490        500        510        520        530        540 
EPALRETDTF DTFMESSWYY ARYCSPQADD ILDPEKANYW LPVDQYVGGI EHACMHLLYS 

       550        560        570        580        590        600 
RFFHKLLRDA GYVTSNEPFK QLLCQGMVLS DAFHHTNEKG TKEWIAPTDV TIERDAKGRI 

       610        620        630        640        650        660 
EKAVDDQGRE VQHSGMIKMS KSKNNGIDPQ EMVDKFGADT VRLFMMFAAP ADMTLEWQES 

       670        680        690        700        710        720 
GVEGASRFLK RVWKLVHEHT TKGTTEALDV AALSGDQKAL RRDVHKTIAK VSDDIGRRQT 

       730        740        750        760        770        780 
FNTAIAAIME LMNKLNKAPQ GSAQDRALLD EALKAVVVML YPMTPHASFA MWEALGESNI 

       790        800        810        820        830        840 
DTTAWPTFDE KALIEDEKTI VVMINGKLRA KLIVAADATE EQVKELGLND ENALKFLDGL 

       850 
TIRKVIYVPG KLLNIVAN 

« Hide

References

[1]"The genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238 shows extensive evidence of gene decay."
Hjerde E., Lorentzen M.S., Holden M.T., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.P., Thomson N.R.
BMC Genomics 9:616-616(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LFI1238.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM178379 Genomic DNA. Translation: CAQ78675.1.
RefSeqYP_002262481.1. NC_011312.1.

3D structure databases

ProteinModelPortalB6EIN2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING316275.VSAL_I0990.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ78675; CAQ78675; VSAL_I0990.
GeneID6986764.
KEGGvsa:VSAL_I0990.
PATRIC20852180. VBIAliSal95923_1117.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAYAANYIL.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ALISL
AccessionPrimary (citable) accession number: B6EIN2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries