B6EIA7 (DXS_ALISL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	VSAL_I0933

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 627	627	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000115717

Sequences

	Sequence		Length	Mass (Da)
	B6EIA7 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 92E775446D318199	FASTA	627	68,703
	10 20 30 40 50 60 MSLDISKYPI LALANTPDEL RSLPKESLPA LCDELRAYLL KSVSKSSGHL ASGLGVVELT 70 80 90 100 110 120 VALHYVYNTP FDQLIWDVGH QAYPHKILTG RREKLSTIRQ KDGLHPFPWR DESEYDVLSV 130 140 150 160 170 180 GHSSTSISAA LGLAICAEKE QANRKVISVI GDGAITAGMA FEALNHAGDI HPDMLVVLND 190 200 210 220 230 240 NEMSISENVG ALNNQLARVL SGSLYTSIRE GGKKVLSGTP TIKELLKRTE EHIKGMVIPG 250 260 270 280 290 300 TMFEELGFNY IGPVDGHDVN ELVRTLKNMR NLKGPQFLHI MTKKGKGYEP AEKDPISYHG 310 320 330 340 350 360 VPKFDPSNHS LPKSSGGKPT FSNIFGDFLC DMAKDDDKLM AITPAMREGS GMVRFSKEFP 370 380 390 400 410 420 GQYFDTAIAE QHAVTLASGM AIAGYNPIVA IYSTFLQRGY DQLIHDVAIM NLPVMFAIDR 430 440 450 460 470 480 AGLVGADGQT HQGAFDISFM RCIPNMVIMT PSDENECRQM LYTGHKHTGP SAVRYPRGSA 490 500 510 520 530 540 TGIEVNKEMQ ALEIGKGRLI RETKITEKGE RVAILNFGTF LSNSVEAAEK LDATVADMRF 550 560 570 580 590 600 AKPLDETLLC ELVTSHDVLV TIEENAISGG AGSGVIEFLM KNRLIKPVLQ LGLPDQFIAQ 610 620 GTQEEMHAEL MLDATGIEKQ IRDYLDL

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LFI1238.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM178379 Genomic DNA. Translation: CAQ78618.1.
RefSeq	YP_002262429.1. NC_011312.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B6EIA7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6986736.
GenomeReviews	Gene locus VSAL_I0933 in contig FM178379_GR.
KEGG	vsa:VSAL_I0933.
PATRIC	20852062. VBIAliSal95923_1058.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	DPILYHG.
ProtClustDB	PRK05444.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ALISL

Accession

Primary (citable) accession number: B6EIA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program