Reviewed, UniProtKB/Swiss-Prot B6EHL7 (SYQ_ALISL)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	VSAL_I0832

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_1000095476
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6EHL7-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 706EECD302DEB027	FASTA	552	63,726
	10 20 30 40 50 60 MSETETRPTN FIRQIIDEDL KSGKHSSVHT RFPPEPNGYL HIGHAKSICL NFGIAQDYQG 70 80 90 100 110 120 QCNLRFDDTN PEKEDIEYVE SIKNDVKWLG FDWSGDIHYS SNYFDKLYDY AVELIEKGLA 130 140 150 160 170 180 YVDELTSDQI REYRGSLKEP GKNSPYRDRS VEENLALFEQ MRDGKFKEGT ICLRAKIDMA 190 200 210 220 230 240 SSFIVMRDPV IYRIRFATHH QTGDKWCIYP MYDFTHCISD ALEGITHSIC TLEFQDNRRL 250 260 270 280 290 300 YDWVLENITI DCQPHQYEFS RLNLEYTIMS KRKLNELVTE KLVNGWDDPR MPTVSGLRRR 310 320 330 340 350 360 GFTAGSIREF CKRIGVTKQE NMIEFGSLES CIRDDLNENA PRAMAVLEPV KVVIENYEEG 370 380 390 400 410 420 KVETLNVANH PNKPEMGTRE VPFTREIYIE QDDFREEANK KYKRLVLGKE VRLRGAYVIQ 430 440 450 460 470 480 ANRIEKDEAG NITTIFCSYD TETLGKNPAD GRKVKGVIHW VSADKAFPAE IRLYDRLFTV 490 500 510 520 530 540 PNSAALEDFS ESINPESLIT KQGFVEPSLV TAEAEAGYQF ERTGYFCIDN KDSTPEALVF 550 NRTVGLRDTW EG

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM178379 Genomic DNA. Translation: CAQ78517.1.
RefSeq	YP_002262339.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6988880.
GenomeReviews	Gene locus VSAL_I0832 in contig FM178379_GR.
KEGG	vsa:VSAL_I0832.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RMPTIAG.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_ALISL

Accession

Primary (citable) accession number: B6EHL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)