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Reviewed, UniProtKB/Swiss-Prot B6EH98 (NRFA_ALISL)

Last modified November 3, 2009. Version 10. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase
Gene names
Name: nrfA
Ordered Locus Names: VSAL_I1997
OrganismAliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]
Taxonomic identifier316275 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

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Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity.

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity.

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the cytochrome c-552 family.

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Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen compound metabolic process

Inferred from electronic annotation. Source: HAMAP

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (cytochrome, ammonia-forming) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 478451Cytochrome c-552 HAMAP MF_01182
PRO_5000405716

Sites

Metal binding911Iron (heme 3 axial ligand) By similarity
Metal binding1231Iron (heme 1 axial ligand) By similarity
Metal binding1611Iron (heme 2 axial ligand) By similarity
Metal binding2101Iron (heme 3 axial ligand) By similarity
Metal binding2121Calcium By similarity
Metal binding2131Calcium; via carbonyl oxygen By similarity
Metal binding2581Calcium; via carbonyl oxygen By similarity
Metal binding2601Calcium By similarity
Metal binding2721Iron (heme 5 axial ligand) By similarity
Metal binding2831Iron (heme 4 axial ligand) By similarity
Metal binding2981Iron (heme 2 axial ligand) By similarity
Metal binding3151Iron (heme 5 axial ligand) By similarity
Metal binding3901Iron (heme 4 axial ligand) By similarity
Binding site1191Heme 1 (covalent) By similarity
Binding site1221Heme 1 (covalent) By similarity
Binding site1571Heme 2 (covalent) By similarity
Binding site1601Heme 2 (covalent) By similarity
Binding site2061Heme 3 (covalent) By similarity
Binding site2091Heme 3 (covalent) By similarity
Binding site2131Substrate By similarity
Binding site2611Substrate By similarity
Binding site2791Heme 4 (covalent) By similarity
Binding site2821Heme 4 (covalent) By similarity
Binding site3111Heme 5 (covalent) By similarity
Binding site3141Heme 5 (covalent) By similarity

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Sequences

Sequence LengthMass (Da)Tools
B6EH98-1 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: D75FA4F1D538F92C

FASTA47853,620
        10         20         30         40         50         60 
MKKQWTRRSA AAIAMVTTLL LSSHSFAASE KTERVDPRNE AYAKQHADQY ESWRPTSDSV 

        70         80         90        100        110        120 
QIEDALAEDP NMVILWAGYG FAKDYNKARG HFYAIDDVRE TLRTAGPTDE NSGPMPMACW 

       130        140        150        160        170        180 
SCKSPDVGRV IEEQGEDGYF SGKWARLGSE IQNPIGCADC HDTRSEEFKN GGPALAITKP 

       190        200        210        220        230        240 
HVERAMNAIG KPFDDQSRLD KQASVCGQCH VEYYFTGKTK AVKFPWDKGT TVDEMEEYYD 

       250        260        270        280        290        300 
EIGFSDWTHK VSKAPMLKAQ HPGYETWREG IHGKNKVVCV DCHMPKVTRE DGTVYTDHKV 

       310        320        330        340        350        360 
GNPFDRFEDT CANCHTQSKD MLREIVSSRK AQVLKMKLTA ERQIVAAHFE AGAAWDAGAT 

       370        380        390        400        410        420 
KEEMAPILQD IRHAQWRWDY AIASHGIHMH APEVALEVLG SAVDRAADAR TKLVRLLAKK 

       430        440        450        460        470 
GITDPIEIPD ISTKAAAQKA LGMDMEGMEA EKKHFLETVV PKWDQQAKER EAADYHPL

« Hide

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References

[1]"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238."
Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

FM178379 Genomic DNA. Translation: CAQ79682.1.
RefSeqYP_002263383.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID6987503.
GenomeReviewsGene locus VSAL_I1997 in contig FM178379_GR.
KEGGvsa:VSAL_I1997.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMACHDQSKE.

Family and domain databases

HAMAPMF_01182.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNRFA_ALISL
AccessionPrimary (citable) accession number: B6EH98
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents