Reviewed, UniProtKB/Swiss-Prot B6EH43 (SYC_ALISL)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	VSAL_I1940

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_1000090814
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP MF_00041
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	211	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Metal binding	240	1	Zinc By similarity
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6EH43-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: B1D639F40EB3A202	FASTA	462	52,334
	10 20 30 40 50 60 MLKIYNSLTR QKEEFKPITE GKVGMYVCGV TIYDLCHIGH GRTFVSFDVI SRYLRFLGYD 70 80 90 100 110 120 LTFVRNITDI DDKIIKRAAE NNESCEALTE RLIAEMHADF DALKMKRPDV EPRATEYITE 130 140 150 160 170 180 IVELVERLIE RGFAYVASNG DVMFEVKKFD EYGRLSRQDL EQLQAGSRVT LEETSVKRSG 190 200 210 220 230 240 MDFVLWKMSK PGEPTWESPW GPGRPGWHIE CSAMNSSILG NHFDIHGGGS DLMFPHHENE 250 260 270 280 290 300 IAQSCCAHDA KYVNTWMHSG MVMIDREKMS KSLGNFFTIR DVLAHYDSET VRYFLMSGHY 310 320 330 340 350 360 RSQLNYSEEN LNQARSSLER LYTSLRGLDL TVTPAGGETF VTRFSTAMND DFNTPEAYSV 370 380 390 400 410 420 LFEMAREVNR LKTENIEAAS KLGALMRELA EVLGLLSQEP EAFLQGDSGS DNEVAEIEAL 430 440 450 460 IKARNDARAA KDWSAADAAR DAITALNIVL EDGPEGTTWR RK

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM178379 Genomic DNA. Translation: CAQ79625.1.
RefSeq	YP_002263332.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6989001.
GenomeReviews	Gene locus VSAL_I1940 in contig FM178379_GR.
KEGG	vsa:VSAL_I1940.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VLWKAAK.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ALISL

Accession

Primary (citable) accession number: B6EH43

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)