Reviewed, UniProtKB/Swiss-Prot B6EGZ6 (GUAA_ALISL)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing]
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase
    GMP synthetase

Gene names

Name:	guaA
Ordered Locus Names:	VSAL_I0738

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP NAD biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_1000120207
Regions
	Domain	9 – 199	191	Glutamine amidotransferase type-1
	Domain	231 – 393	163	GMP-binding
	Nucleotide binding	227 – 233	7	ATP By similarity
Sites
	Active site	86	1	Nucleophile By similarity
	Active site	173	1	By similarity
	Active site	175	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6EGZ6-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 53795B7F536CBE1D	FASTA	517	57,421
	10 20 30 40 50 60 MTTNIHAQRI LILDFGSQYT QLVARRIREI GVYCELWSWD VEESDIRDFN PDGIILSGGP 70 80 90 100 110 120 ESVTEANSPR APQYVFDSGV PVFGVCYGMQ TMAEQLGGKV ATSTEREFGY AAVQVTGESA 130 140 150 160 170 180 LFKDLEATQD VWMSHGDKVV EIPSDFVKIA ETETCPYAAM ANEEKKYYGV QFHPEVTHTK 190 200 210 220 230 240 NGLKMLENFV LNVCGCEGLW TSASIIEDAI ARIKEQVGDD EVILGLSGGV DSSVVAMLAH 250 260 270 280 290 300 RAIGDKLTCV FVDNGLLRLN EAEQVMEMFS EGFGLKIIHV EAEERFLSAL SGESDPEAKR 310 320 330 340 350 360 KIIGHVFVDI FDEESKKLSN AKWLAQGTIY PDVIESAASK TGKAHVIKSH HNVGGLPDDM 370 380 390 400 410 420 EMGLVEPLRE LFKDEVRKIG LELGLPYNML YRHPFPGPGL GVRVLGEVKK EYCDLLRRAD 430 440 450 460 470 480 AIFIEELHNA DLYNKVSQAF TVFLPVRSVG VMGDGRKYDW VVSLRAVETI DFMTAHWAHL 490 500 510 PYDFLGKVSN RIINEVGGIS RVVYDISGKP PATIEWE

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	FM178379 Genomic DNA. Translation: CAQ78423.1.
RefSeq	YP_002262255.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6988634.
GenomeReviews	Gene locus VSAL_I0738 in contig FM178379_GR.
KEGG	vsa:VSAL_I0738.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DQVMEMF.
Family and domain databases
HAMAP	MF_00344. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit. TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_ALISL

Accession

Primary (citable) accession number: B6EGZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)