B6EGC1 (TRMD_ALISL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	VSAL_I0651

Organism

Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) [Complete proteome] [HAMAP]

Taxonomic identifier

316275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000130126
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	113	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6EGC1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 1E095E782EBA92AC	FASTA	249	27,789
	10 20 30 40 50 60 MWVGVISLFP EMFRSVTDFG VTSQAIKKGL LSIETWNPRD FTQDKHRTVD DRPYGGGPGM 70 80 90 100 110 120 LMMVQPLRDA ITAAREAAPG KTKVIYLSPQ GRTLNQAGVE ELATNENLVL ICGRYEGVDE 130 140 150 160 170 180 RIIQSEVDEE WSIGDFVLTG GELPAMTLID SVSRFVPGVL GDFASAEEDS FADGLLDCPH 190 200 210 220 230 240 YTRPEVLDGK EVPSVLKSGN HKDIARWRMK QSLGRTWLRR PELLGNLALT DEQELLLAEF VREEHQQSK

References

[1]

"The complete genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238." Hjerde E., Lorentzen M.S., Holden M.T.G., Seeger K., Paulsen S., Bason N., Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S., Thurston S., Parkhill J., Willassen N.-P., Thomson N.R. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LFI1238.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM178379 Genomic DNA. Translation: CAQ78336.1.
RefSeq	YP_002262170.1. NC_011312.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B6EGC1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6989106.
GenomeReviews	Gene locus VSAL_I0651 in contig FM178379_GR.
KEGG	vsa:VSAL_I0651.
PATRIC	20851432. VBIAliSal95923_0761.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285805.
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ALISL

Accession

Primary (citable) accession number: B6EGC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program