B6D434 (CAMP_BUNFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cathelicidin-BF antimicrobial peptide Alternative name(s): Cathelicidin-related protein
Organism	Bungarus fasciatus (Banded krait)
Taxonomic identifier	8613 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Bungarinae › Bungarus

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potent antimicrobial peptide against most of Gram-negative bacteria, some Gram-positive bacteria (Bacillus) and some fungi (C.albicans, P.pastoris, A.terreus, A.nidulans, and C.globosum). Adopts an amphipathic alpha helical conformation, that may allow to partition into the target membrane. No hemolytic and cytotoxic activities have been observed on mammalian cells. Ref.1
Subcellular location	Secreted. Target cell membrane Probable Ref.1.
Tissue specificity	Expressed by the venom gland, stomach, trachea, skin, muscle, heart, kidney, lung, brain, intestine, spleen, liver, and ovary. Ref.1
Miscellaneous	A predicted cleavage site is located at position Val-157-158-Lys, giving a peptide of 34 residues instead of the 30 residues found for the purified protein. The 30 residues cathelicidin-BF could result from a further processing.
Sequence similarities	Belongs to the cathelicidin family.
Mass spectrometry	Molecular mass is 3638.0±0.5 Da from positions 162 - 191. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Immunity
Cellular component	Membrane Secreted Target cell membrane Target membrane
Domain	Signal
Molecular function	Antibiotic Antimicrobial Fungicide
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW killing of cells of other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-KW other organism cell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 161	139			PRO_0000410956
	Peptide	162 – 191	30	Cathelicidin-BF antimicrobial peptide Ref.1		PRO_0000410957
Regions
	Compositional bias	125 – 150	26	Glu-rich
	Compositional bias	151 – 181	31	Lys-rich
Amino acid modifications
	Disulfide bond	81 ↔ 92		By similarity
	Disulfide bond	103 ↔ 120		By similarity

Sequences

	Sequence		Length	Mass (Da)
	B6D434 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: A8D667E4D6ACBE9A	FASTA	191	21,870
	10 20 30 40 50 60 MEGFFWKTLL VVGALAIAGT SSLPHKPLIY EEAVDLAVSI YNSKSGEDSL YRLLEAVSPP 70 80 90 100 110 120 KWDPLSESNQ ELNFTMKETV CLVAEERSLE ECDFQEDGVV MGCTGYYFFG ESPPVVVLTC 130 140 150 160 170 180 KPVGEEGEQK QEEGNEEEKE VEEEEQEEDE KDQPRRVKRF KKFFRKLKKS VKKRAKEFFK 190 KPRVIGVSIP F

References

[1]

"Snake cathelicidin from Bungarus fasciatus is a potent peptide antibiotics." Wang Y., Hong J., Liu X., Yang H., Liu R., Wu J., Wang A., Lin D., Lai R. PLoS ONE 3:E3217-E3217(2008) [PubMed: 18795096] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 162-191, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, CIRCULAR DICHROISM, MASS SPECTROMETRY, SYNTHESIS OF 157-171 AND 162-191. Tissue: Venom and Venom gland.

[2]

"Identification and characterization of novel reptile cathelicidins from elapid snakes." Zhao H., Gan T.-X., Liu X.-D., Jin Y., Lee W.-H., Shen J.-H., Zhang Y. Peptides 29:1685-1691(2008) [PubMed: 18620012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU753183 mRNA. Translation: ACI22652.1. EU622893 mRNA. Translation: ACF21001.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG105653.
Family and domain databases
InterPro	IPR001894. Cathelicidin. [Graphical view]
PANTHER	PTHR10206. Cathelicidin. 1 hit.
Pfam	PF00666. Cathelicidins. 1 hit. [Graphical view]
ProDom	PD001838. Cathelicidin. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00946. CATHELICIDINS_1. False negative. PS00947. CATHELICIDINS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAMP_BUNFA

Accession

Primary (citable) accession number: B6D434

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	November 25, 2008
Last modified:	July 27, 2011
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program