B6CZ62 (TOMT_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane O-methyltransferase
EC=2.1.1.6

Alternative name(s):
Catechol O-methyltransferase 2
Protein LRTOMT2

Gene names

Name:	Tomt
Synonyms:	Comt2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Required for auditory function By similarity. UniProtKB P21964 UniProtKB Q8WZ04
Catalytic activity	S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol. UniProtKB P21964
Subcellular location	Cytoplasm By similarity UniProtKB A1Y9I9.
Miscellaneous	In primates, this protein is produced by a bicistronic gene which also produces the LRRC51 protein from an overlapping reading frame. In rodents, these proteins are produced by 2 separate adjacent genes which together are orthologous to the single primate gene. Ref.1
Sequence similarities	Belongs to the methyltransferase superfamily. Catechol-O-methyltransferase family.
Caution	Despite its name, the rat TOMT protein does not contain a transmembrane region in contrast to primate orthologs.

Ontologies

Keywords
Biological process	Catecholamine metabolism Hearing Neurotransmitter degradation
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	auditory receptor cell development Inferred from electronic annotation. Source: Compara catecholamine catabolic process Inferred from electronic annotation. Source: Compara neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of sound Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	catechol O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Transmembrane O-methyltransferase		PRO_0000372489

Sequences

	Sequence		Length	Mass (Da)
	B6CZ62 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: AF1EFE98697A0B42	FASTA	258	28,864
	10 20 30 40 50 60 MSPAIALAFL PLVVTLLVRY RHHFRLLVRT VLLRSFRDCL SGLRIEERAF SYVITHALPG 70 80 90 100 110 120 DPGHILTTLD HWSSCCEYLS HMGPIKGQIL MRLVEEKAPA CVLELGTHCG YSTLLIARAL 130 140 150 160 170 180 PPGSRLLTVE RDSRTAAVAE KVIRLAGFDE QMVELIAGSS EEVIPRLRAQ HQLNRADLVL 190 200 210 220 230 240 LAHRPRYYLR DLQLLEAHAL LPHGATVLAD HVLFPGAPRF LQYTKSCGRY RCRLHHTSLP 250 DFPAIKDGIA QLTYTGPG

References

[1]

"Mutations of LRTOMT, a fusion gene with alternative reading frames, cause nonsyndromic deafness in humans." Ahmed Z.M., Masmoudi S., Kalay E., Belyantseva I.A., Mosrati M.A., Collin R.W.J., Riazuddin S., Hmani-Aifa M., Venselaar H., Kawar M.N., Tlili A., van der Zwaag B., Khan S.Y., Ayadi L., Riazuddin S.A., Morell R.J., Griffith A.J., Charfedine I. , Caylan R., Oostrik J., Karaguzel A., Ghorbel A., Riazuddin S., Friedman T.B., Ayadi H., Kremer H. Nat. Genet. 40:1335-1340(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU627093 mRNA. Translation: ACF40903.1.
IPI	IPI00368471.
RefSeq	NP_001132966.1. NM_001139494.1.
UniGene	Rn.220283.
3D structure databases
ProteinModelPortal	B6CZ62.
ModBase	Search...
Proteomic databases
PaxDb	B6CZ62.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000039564; ENSRNOP00000036144; ENSRNOG00000023434.
GeneID	308868.
KEGG	rno:308868.
Organism-specific databases
CTD	220074.
RGD	1561509. Tomt.
Phylogenomic databases
eggNOG	COG4122.
GeneTree	ENSGT00390000011316.
HOGENOM	HOG000046392.
OrthoDB	EOG44QT1M.
Gene expression databases
Genevestigator	B6CZ62.
Family and domain databases
InterPro	IPR025782. Catechol_O-MeTrfase. IPR002935. O-MeTrfase_3. [Graphical view]
PANTHER	PTHR10509. PTHR10509. 1 hit.
Pfam	PF01596. Methyltransf_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	659690.

Entry information

Entry name

TOMT_RAT

Accession

Primary (citable) accession number: B6CZ62

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	November 25, 2008
Last modified:	April 3, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program