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Protein

Transmembrane O-methyltransferase homolog

Gene

Tomt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones (By similarity). Required for auditory function (By similarity). Component of the cochlear hair cell's mechanotransduction (MET) machinery. Involved in the assembly of the asymmetric tip-link MET complex. Required for transportation of TMC1 and TMC2 proteins into the mechanically sensitive stereocilia of the hair cells. The function in MET is independent of the enzymatic activity (By similarity).By similarity

Miscellaneous

In primates, this protein is produced by a bicistronic gene which also produces the LRRC51 protein from an overlapping reading frame. In rodents, these proteins are produced by 2 separate adjacent genes which together are orthologous to the single primate gene.

Caution

Despite its name, the rat TOMT protein does not contain a transmembrane region in contrast to primate orthologs.Curated

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei112S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei130S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei160S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processCatecholamine metabolism, Hearing, Neurotransmitter degradation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-RNO-379397 Enzymatic degradation of dopamine by COMT

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane O-methyltransferase homologImported (EC:2.1.1.6By similarity)
Alternative name(s):
Catechol O-methyltransferase 2By similarity
Protein LRTOMT21 Publication
Gene namesi
Name:TomtImported
Synonyms:Comt2By similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1561509 Tomt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Keywords - Diseasei

Deafness

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003724891 – 258Transmembrane O-methyltransferase homologAdd BLAST258

Proteomic databases

PaxDbiB6CZ62

PTM databases

PhosphoSitePlusiB6CZ62

Expressioni

Gene expression databases

BgeeiENSRNOG00000023434

Interactioni

Subunit structurei

Interacts with LHFPL5, PCDH15, TMC1, TMC2 and TMIE. Interacts directly with TMC1. The interaction of TOMT with TMC1 and TMC2 is required for the transportation of TMC1/2 into the stereocilia of hair cells.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000036144

Structurei

3D structure databases

ProteinModelPortaliB6CZ62
SMRiB6CZ62
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 107S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1663 Eukaryota
COG4122 LUCA
GeneTreeiENSGT00390000011316
HOGENOMiHOG000046392
InParanoidiB6CZ62
KOiK00545
OMAiFIDHYKP
OrthoDBiEOG091G0GQK
PhylomeDBiB6CZ62
TreeFamiTF329140

Family and domain databases

InterProiView protein in InterPro
IPR002935 O-MeTrfase_3
IPR029063 SAM-dependent_MTases
IPR033025 TOMT
PANTHERiPTHR43836:SF1 PTHR43836:SF1, 1 hit
PfamiView protein in Pfam
PF01596 Methyltransf_3, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51682 SAM_OMT_I, 1 hit

Sequencei

Sequence statusi: Complete.

B6CZ62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPAIALAFL PLVVTLLVRY RHHFRLLVRT VLLRSFRDCL SGLRIEERAF
60 70 80 90 100
SYVITHALPG DPGHILTTLD HWSSCCEYLS HMGPIKGQIL MRLVEEKAPA
110 120 130 140 150
CVLELGTHCG YSTLLIARAL PPGSRLLTVE RDSRTAAVAE KVIRLAGFDE
160 170 180 190 200
QMVELIAGSS EEVIPRLRAQ HQLNRADLVL LAHRPRYYLR DLQLLEAHAL
210 220 230 240 250
LPHGATVLAD HVLFPGAPRF LQYTKSCGRY RCRLHHTSLP DFPAIKDGIA

QLTYTGPG
Length:258
Mass (Da):28,864
Last modified:November 25, 2008 - v1
Checksum:iAF1EFE98697A0B42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU627093 mRNA Translation: ACF40903.1
RefSeqiNP_001132966.1, NM_001139494.1
XP_017444679.1, XM_017589190.1
XP_017444680.1, XM_017589191.1
UniGeneiRn.220283

Genome annotation databases

EnsembliENSRNOT00000039564; ENSRNOP00000036144; ENSRNOG00000023434
GeneIDi308868
KEGGirno:308868

Similar proteinsi

Entry informationi

Entry nameiTOMT_RAT
AccessioniPrimary (citable) accession number: B6CZ62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 25, 2008
Last modified: April 25, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health