B6CZ56 (TOMT_PROCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane O-methyltransferase
EC=2.1.1.6

Alternative name(s):
Catechol O-methyltransferase 2
Protein LRTOMT2

Gene names

Name:	LRTOMT
Synonyms:	COMT2, TOMT

Organism

Propithecus coquereli (Coquerel's sifaka) (Propithecus verreauxi coquereli)

Taxonomic identifier

379532 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Strepsirrhini › Lemuriformes › Indriidae › Propithecus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Required for auditory function By similarity. UniProtKB Q8WZ04 UniProtKB P21964
Catalytic activity	S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol. UniProtKB P21964
Subcellular location	Isoform 1: Membrane; Single-pass membrane protein Potential. Isoform 2: Cytoplasm By similarity UniProtKB A1Y9I9.
Miscellaneous	In primates, this protein is produced by a bicistronic gene which also produces the LRRC51 protein from an overlapping reading frame. In rodents, these proteins are produced by 2 separate adjacent genes which together are orthologous to the single primate gene. Ref.1
Sequence similarities	Belongs to the methyltransferase superfamily. Catechol-O-methyltransferase family.
Sequence caution	The sequence ACF40897.1 differs from that shown. Reason: Frameshift at position 12.

Ontologies

Keywords
Biological process	Catecholamine metabolism Hearing Neurotransmitter degradation
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	catecholamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of sound Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	catechol O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: B6CZ56-1)

Also known as: C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: B6CZ56-2)

Also known as: D;

The sequence of this isoform differs from the canonical sequence as follows:
     12-102: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Transmembrane O-methyltransferase		PRO_0000372488
Regions
	Transmembrane	14 – 34	21	Helical; Potential
Natural variations
	Alternative sequence	12 – 102	91	Missing in isoform 2. Ref.1		VSP_053069

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (C) [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 12B1949D04FC4107	FASTA	274	30,432
	10 20 30 40 50 60 MGTPWRKRKG ITQVGTMSPA IALAFLPLVV TLLVRYRHYF RLLVGTVLLR SLRDCLSGLR 70 80 90 100 110 120 IEERAFSYVL THALPGDPGH ILTTLDHWSS HCEYLSHMGP VKGQILMRLV EEKAPACVLE 130 140 150 160 170 180 LGTYCGYSTL LIAQALPPGG RLLTVERDPR TAAVAEKLIR LAGFDEHMVE LIVGSSEEVI 190 200 210 220 230 240 PCLRTQYQLS RADLVLLIHR PRCYLRDLQL LEAHALLPAG ATVLADHVLF PGAPRFLQYA 250 260 270 KSCGRYRCRL YHTGLPDFPA IKDGIAQLTY AGPG
	Isoform 2 (D) [UniParc]. Checksum: 036F313CF54A0235	FASTA	183	20,221
	10 20 30 40 50 60 MGTPWRKRKG IGQILMRLVE EKAPACVLEL GTYCGYSTLL IAQALPPGGR LLTVERDPRT 70 80 90 100 110 120 AAVAEKLIRL AGFDEHMVEL IVGSSEEVIP CLRTQYQLSR ADLVLLIHRP RCYLRDLQLL 130 140 150 160 170 180 EAHALLPAGA TVLADHVLFP GAPRFLQYAK SCGRYRCRLY HTGLPDFPAI KDGIAQLTYA GPG

References

[1]

"Mutations of LRTOMT, a fusion gene with alternative reading frames, cause nonsyndromic deafness in humans." Ahmed Z.M., Masmoudi S., Kalay E., Belyantseva I.A., Mosrati M.A., Collin R.W.J., Riazuddin S., Hmani-Aifa M., Venselaar H., Kawar M.N., Tlili A., van der Zwaag B., Khan S.Y., Ayadi L., Riazuddin S.A., Morell R.J., Griffith A.J., Charfedine I. , Caylan R., Oostrik J., Karaguzel A., Ghorbel A., Riazuddin S., Friedman T.B., Ayadi H., Kremer H. Nat. Genet. 40:1335-1340(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU627087 mRNA. Translation: ACF40897.1. Frameshift. EU627088 mRNA. Translation: ACF40898.1.
3D structure databases
ProteinModelPortal	B6CZ56.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR025782. Catechol_O-MeTrfase. IPR002935. O-MeTrfase_3. [Graphical view]
PANTHER	PTHR10509. PTHR10509. 1 hit.
Pfam	PF01596. Methyltransf_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOMT_PROCO

Accession

Primary (citable) accession number: B6CZ56
Secondary accession number(s): B6CZ57

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	October 3, 2012
This is version 15 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program