Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B6CZ46 (TOMT_MACMU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transmembrane O-methyltransferase

EC=2.1.1.6
Alternative name(s):
Catechol O-methyltransferase 2
Protein LRTOMT2
Gene names
Name:LRTOMT
Synonyms:COMT2, TOMT
OrganismMacaca mulatta (Rhesus macaque) [Reference proteome]
Taxonomic identifier9544 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Required for auditory function By similarity. UniProtKB Q8WZ04 UniProtKB P21964

Catalytic activity

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol. UniProtKB P21964

Subcellular location

Isoform 1: Membrane; Single-pass membrane protein Potential.

Isoform 2: Cytoplasm By similarity UniProtKB A1Y9I9.

Miscellaneous

In primates, this protein is produced by a bicistronic gene which also produces the LRRC51 protein from an overlapping reading frame. In rodents, these proteins are produced by 2 separate adjacent genes which together are orthologous to the single primate gene. Ref.1

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.

Sequence caution

The sequence ACF40887.1 differs from that shown. Reason: Frameshift at positions 18 and 31.

The sequence ACF40888.1 differs from that shown. Reason: Frameshift at position 11.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: B6CZ46-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.1 (identifier: B6CZ46-2)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     11-32: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296Transmembrane O-methyltransferase
PRO_0000372486

Regions

Transmembrane36 – 5621Helical; Potential
Region144 – 1452S-adenosyl-L-methionine binding By similarity

Sites

Binding site1421S-adenosyl-L-methionine By similarity
Binding site1501S-adenosyl-L-methionine By similarity
Binding site1681S-adenosyl-L-methionine By similarity
Binding site1981S-adenosyl-L-methionine; via amide nitrogen By similarity

Natural variations

Alternative sequence11 – 3222Missing in isoform 2. Ref.1
VSP_053068

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (B) [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 9CE89610D5E5EF93

FASTA29632,666
        10         20         30         40         50         60 
MGTPWRKRKG IAGPGLPNLS CALVLCLSRS QPRTQVGTMS PAIALAFLPL VVTLLVRYRH 

        70         80         90        100        110        120 
YFRLLVGTVL LRSLRDCLSG LRIEERAFSY VLTHALPGDP GHILTTLDHW SSHCEYLSHM 

       130        140        150        160        170        180 
GPVKGQILMR LVEEKAPACV LELGTYCGYS TLLIAQALPP GGRLLTVERD PRTAAVAEKL 

       190        200        210        220        230        240 
IRLAGFDEHM VELIVGSSEE VIPCLRTQYQ LSRADLVLLI HRPRCYLRDL QLLEAHALLP 

       250        260        270        280        290 
AGATVLADHV LFPGAPRFLQ YAKSCGRYRC RLYHTGLPDF PAIKDGIAQL TYAGPG 

« Hide

Isoform 2 (C) [UniParc].

Checksum: 641A9491B2EC4102
Show »

FASTA27430,475

References

[1]"Mutations of LRTOMT, a fusion gene with alternative reading frames, cause nonsyndromic deafness in humans."
Ahmed Z.M., Masmoudi S., Kalay E., Belyantseva I.A., Mosrati M.A., Collin R.W.J., Riazuddin S., Hmani-Aifa M., Venselaar H., Kawar M.N., Tlili A., van der Zwaag B., Khan S.Y., Ayadi L., Riazuddin S.A., Morell R.J., Griffith A.J., Charfedine I. expand/collapse author list , Caylan R., Oostrik J., Karaguzel A., Ghorbel A., Riazuddin S., Friedman T.B., Ayadi H., Kremer H.
Nat. Genet. 40:1335-1340(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU627077 mRNA. Translation: ACF40887.1. Frameshift.
EU627078 mRNA. Translation: ACF40888.1. Frameshift.
RefSeqNP_001129574.1. NM_001136102.1.
UniGeneMmu.17727.

3D structure databases

ProteinModelPortalB6CZ46.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID718286.
KEGGmcc:718286.

Organism-specific databases

CTD220074.

Family and domain databases

InterProIPR025782. Catechol_O-MeTrfase.
IPR002935. O-MeTrfase_3.
[Graphical view]
PANTHERPTHR10509. PTHR10509. 1 hit.
PfamPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PROSITEPS51682. SAM_OMT_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio19970910.

Entry information

Entry nameTOMT_MACMU
AccessionPrimary (citable) accession number: B6CZ46
Secondary accession number(s): B6CZ47
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: February 19, 2014
This is version 25 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families