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Protein

Transmembrane O-methyltransferase

Gene

LRTOMT

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones (By similarity). Required for auditory function (By similarity). Component of the cochlear hair cell's mechanotransduction (MET) machinery. Involved in the assembly of the asymmetric tip-link MET complex. Required for transportation of TMC1 and TMC2 proteins into the mechanically sensitive stereocilia of the hair cells. The function in MET is independent of the enzymatic activity (By similarity).By similarity

Miscellaneous

In primates, this protein is produced by a bicistronic gene which also produces the LRRC51 protein from an overlapping reading frame. In rodents, these proteins are produced by 2 separate adjacent genes which together are orthologous to the single primate gene.

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei150S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei168S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei198S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processCatecholamine metabolism, Hearing, Neurotransmitter degradation
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane O-methyltransferaseBy similarity (EC:2.1.1.6By similarity)
Alternative name(s):
Catechol O-methyltransferase 2By similarity
Protein LRTOMT21 Publication
Gene namesi
Name:LRTOMT1 Publication
Synonyms:COMT2By similarity, TOMTBy similarity
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
Proteomesi
  • UP000006718 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Keywords - Diseasei

Deafness

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003724861 – 296Transmembrane O-methyltransferaseAdd BLAST296

Interactioni

Subunit structurei

Interacts with LHFPL5, PCDH15, TMC1, TMC2 and TMIE. Interacts directly with TMC1. The interaction of TOMT with TMC1 and TMC2 is required for the transportation of TMC1/2 into the stereocilia of hair cells.By similarity

Structurei

3D structure databases

ProteinModelPortaliB6CZ46
SMRiB6CZ46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni144 – 145S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiB6CZ46

Family and domain databases

InterProiView protein in InterPro
IPR002935 O-MeTrfase_3
IPR029063 SAM-dependent_MTases
IPR033025 TOMT
PANTHERiPTHR43836:SF1 PTHR43836:SF1, 1 hit
PfamiView protein in Pfam
PF01596 Methyltransf_3, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51682 SAM_OMT_I, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: B6CZ46-1) [UniParc]FASTAAdd to basket
Also known as: B1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTPWRKRKG IAGPGLPNLS CALVLCLSRS QPRTQVGTMS PAIALAFLPL
60 70 80 90 100
VVTLLVRYRH YFRLLVGTVL LRSLRDCLSG LRIEERAFSY VLTHALPGDP
110 120 130 140 150
GHILTTLDHW SSHCEYLSHM GPVKGQILMR LVEEKAPACV LELGTYCGYS
160 170 180 190 200
TLLIAQALPP GGRLLTVERD PRTAAVAEKL IRLAGFDEHM VELIVGSSEE
210 220 230 240 250
VIPCLRTQYQ LSRADLVLLI HRPRCYLRDL QLLEAHALLP AGATVLADHV
260 270 280 290
LFPGAPRFLQ YAKSCGRYRC RLYHTGLPDF PAIKDGIAQL TYAGPG
Length:296
Mass (Da):32,666
Last modified:May 5, 2009 - v2
Checksum:i9CE89610D5E5EF93
GO
Isoform 21 Publication (identifier: B6CZ46-2) [UniParc]FASTAAdd to basket
Also known as: C1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     11-32: Missing.

Show »
Length:274
Mass (Da):30,475
Checksum:i641A9491B2EC4102
GO

Sequence cautioni

The sequence ACF40887 differs from that shown. Reason: Frameshift at positions 18 and 31.Curated
The sequence ACF40888 differs from that shown. Reason: Frameshift at position 11.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05306811 – 32Missing in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU627077 mRNA Translation: ACF40887.1 Frameshift.
EU627078 mRNA Translation: ACF40888.1 Frameshift.
RefSeqiNP_001129574.1, NM_001136102.1
UniGeneiMmu.17727

Genome annotation databases

GeneIDi718286

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTOMT_MACMU
AccessioniPrimary (citable) accession number: B6CZ46
Secondary accession number(s): B6CZ47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: April 25, 2018
This is version 40 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health