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B6CZ18

- GRK7B_XENLA

UniProt

B6CZ18 - GRK7B_XENLA

Protein

G protein-coupled receptor kinase 7B

Gene

grk7-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).By similarity

    Catalytic activityi

    ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
    ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei218 – 2181ATPPROSITE-ProRule annotation
    Active sitei314 – 3141Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi195 – 2039ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
    3. rhodopsin kinase activity Source: UniProtKB

    GO - Biological processi

    1. signal transduction Source: InterPro
    2. termination of G-protein coupled receptor signaling pathway Source: InterPro
    3. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-coupled receptor kinase 7B (EC:2.7.11.14, EC:2.7.11.16)
    Gene namesi
    Name:grk7-b
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-6252064. grk7.

    Subcellular locationi

    Membrane By similarity; Lipid-anchor By similarity

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 547547G protein-coupled receptor kinase 7BPRO_0000412818Add
    BLAST
    Propeptidei548 – 5503Removed in mature formBy similarityPRO_0000412819

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361Phosphoserine1 Publication
    Modified residuei487 – 4871PhosphoserineBy similarity
    Modified residuei547 – 5471Cysteine methyl esterSequence Analysis
    Lipidationi547 – 5471S-geranylgeranyl cysteineSequence Analysis

    Post-translational modificationi

    Autophosphorylated in vitro at Ser-487 By similarity. Phosphorylation at Ser-36 is regulated by light and activated by cAMP.By similarity1 Publication

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Expressioni

    Tissue specificityi

    Retina, cones.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliB6CZ18.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini57 – 174118RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini189 – 451263Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini452 – 51766AGC-kinase C-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG004532.
    KOiK00909.

    Family and domain databases

    InterProiIPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR00717. GPCRKINASE.
    SMARTiSM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B6CZ18-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCDMGGLDNL IANTAYLQAR KSSEGDAKEL QKRRKSLSLP PPDVSRNEVK    50
    ETITLDYQSI CVEQPIGQRL FKDFLATVPE YKLAEDFLEE VKEWELEEGS 100
    AKEQLMEKLV SRRFKEPAEG SLNFLGKDLS SRIQQAQSKD MPELILLAKD 150
    SGNAFLMDAP FQDFQNSPFY DRFLQWKAFE RQPINQKYFY EFRILGKGGF 200
    GEVCAIQVKN TGQMYACKKL DKKRLKKKNG EKMALLEKEI LEKVHSPFIV 250
    SLAYAYETKT HLCLVMSLMN GGDLKFHIYN VGDKGVEIKR VIFYSAQICC 300
    GILHLHSLKI VYRDMKPENV LLDDHGNCRL SDLGLAVKVK EGKAITQRAG 350
    TNGYMAPEIL TDEDYSYPVD WFAMGCSIFE MIAAYTPFRD PKEKTSKEEL 400
    KRKTLEDEVV FPLPTFTEEA KDICRLFLAK KPQNRLGSRT NDDDPRKHAF 450
    FKSINFQRLE AGMVDPPFVP DPSVVYAKDI SDIADFSEVK GIEFDDKDAK 500
    LFKRFSTGAV PISWQKEIID TGLFDELNDP SREVTGGGNS GEKSGVCSIL 550
    Length:550
    Mass (Da):62,493
    Last modified:November 25, 2008 - v1
    Checksum:i86F610D8275855CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU621674 mRNA. Translation: ACF28431.1.
    RefSeqiNP_001131052.1. NM_001137580.1.
    UniGeneiXl.85104.

    Genome annotation databases

    GeneIDi100192359.
    KEGGixla:100192359.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU621674 mRNA. Translation: ACF28431.1 .
    RefSeqi NP_001131052.1. NM_001137580.1.
    UniGenei Xl.85104.

    3D structure databases

    ProteinModelPortali B6CZ18.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100192359.
    KEGGi xla:100192359.

    Organism-specific databases

    CTDi 100192359.
    Xenbasei XB-GENE-6252064. grk7.

    Phylogenomic databases

    HOVERGENi HBG004532.
    KOi K00909.

    Family and domain databases

    InterProi IPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00717. GPCRKINASE.
    SMARTi SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Phosphorylation of GRK7 by PKA in cone photoreceptor cells is regulated by light."
      Osawa S., Jo R., Weiss E.R.
      J. Neurochem. 107:1314-1324(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-36.

    Entry informationi

    Entry nameiGRK7B_XENLA
    AccessioniPrimary (citable) accession number: B6CZ18
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 21, 2011
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3