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B6CZ17 (GRK7A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G protein-coupled receptor kinase 7A

EC=2.7.11.14
EC=2.7.11.16
Gene names
Name:grk7-a
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO) By similarity.

Catalytic activity

ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Subcellular location

Membrane; Lipid-anchor By similarity.

Post-translational modification

Autophosphorylated in vitro at Ser-487 By similarity. Phosphorylation at Ser-36 is regulated by light and activated by cAMP. Ref.1

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 548548G protein-coupled receptor kinase 7A
PRO_0000412816
Propeptide549 – 5513Removed in mature form By similarity
PRO_0000412817

Regions

Domain57 – 174118RGS
Domain189 – 451263Protein kinase
Domain452 – 51766AGC-kinase C-terminal
Nucleotide binding195 – 2039ATP By similarity
Compositional bias536 – 5394Poly-Gly

Sites

Active site3141Proton acceptor By similarity
Binding site2181ATP By similarity

Amino acid modifications

Modified residue361Phosphoserine Ref.1
Modified residue4871Phosphoserine By similarity
Modified residue5481Cysteine methyl ester Potential
Lipidation5481S-geranylgeranyl cysteine Potential

Sequences

Sequence LengthMass (Da)Tools
B6CZ17 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 87140A98514D7022

FASTA55162,693
        10         20         30         40         50         60 
MCDMGGLDNL IANTAYLQAR KNSEGDVKEL QKRRKSLSLP STDVSRKEIK ETITLDYQSI 

        70         80         90        100        110        120 
CVEQPIGQRL FRDFLGTVSE YKLAEEFLEE VKEWELEEGS AKEQLMEKLV SRRFKEPAEG 

       130        140        150        160        170        180 
SLNFLGKDLS SRIQQAQSKD MPELILLAKD AGNAFLMDAP FQDFQNSPFY DRFLQWKAFE 

       190        200        210        220        230        240 
RQPINQKYFY EFRILGKGGF GEVCAIQVKN TGQMYACKKL DKKRLKKKNG EKMALLEKEI 

       250        260        270        280        290        300 
LEKVHSPFIV SLAYAYETKT HLCLVMSLMN GGDLKFHIYN IGEKGIEIKR VIFYSAQICC 

       310        320        330        340        350        360 
GILHLHSLKI LYRDMKPENV LLDDNGNCRL SDLGLAVKVK EGKPITQRAG TNGYMAPEIL 

       370        380        390        400        410        420 
TDVDYSYPVD WFAMGCSIYE MIAGHTPFRD PKEKTSKEEV KRKTIEDEVV FQHPVFTEEA 

       430        440        450        460        470        480 
KDICRLFLAK KPQNRLGSRT NDDDPRKHAF FKSINFQRLE AGMVDPPFVP DPSVVYAKDI 

       490        500        510        520        530        540 
SDIADFSEVK GIEFDDKDSK FFKRFATGAI PISWQKEIID TGLFDELNDP SREATGGGGN 

       550 
SGEKSGVCSI L 

« Hide

References

[1]"Phosphorylation of GRK7 by PKA in cone photoreceptor cells is regulated by light."
Osawa S., Jo R., Weiss E.R.
J. Neurochem. 107:1314-1324(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT SER-36.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU621673 mRNA. Translation: ACF28430.1.
RefSeqNP_001131051.1. NM_001137579.1.
UniGeneXl.85028.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100192358.
KEGGxla:100192358.

Organism-specific databases

CTD100192358.
XenbaseXB-GENE-6055238. grk7.

Phylogenomic databases

HOVERGENHBG004532.
KOK00909.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR00717. GPCRKINASE.
SMARTSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGRK7A_XENLA
AccessionPrimary (citable) accession number: B6CZ17
Entry history
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: November 25, 2008
Last modified: February 19, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families