B6A497 (B6A497_RHILW) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 3 HAMAP MF_00444
EC=3.4.21.92 HAMAP MF_00444

Alternative name(s):
Endopeptidase Clp 3 HAMAP MF_00444

Gene names

Name:	clpP3 HAMAP MF_00444
Ordered Locus Names:	Rleg2_6060

Encoded on

Plasmid pRLG202 EMBL ACI59230.1

Organism

Rhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP]

Taxonomic identifier

395492 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family. RuleBase RU003567 HAMAP MF_00444

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00444
Ligand	ATP-binding HAMAP MF_00444 Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease HAMAP MF_00444
Technical term	Complete proteome Plasmid EMBL ACI59230.1
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	102	1	By similarity HAMAP MF_00444
	Active site	127	1	By similarity HAMAP MF_00444

Sequences

	Sequence		Length	Mass (Da)
	B6A497 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 42A768D67C8888AD	FASTA	203	22,968
	10 20 30 40 50 60 MREAMQLVPM VVEQSSRGER SFDIYSRLLR ERIVFLNGEV NDTVSALVCA QLLFLEAENP 70 80 90 100 110 120 KKPIYLYINS PGGVVTSGLA MYDTMRYIRA PVHTLCMGTA RSMGSFLLMA GEPGERAALP 130 140 150 160 170 180 NASILIHQPS GGFQGQATDM QIHAEEILKT KQRMTRLYAE HCGRSYEDFE RAMDRDRFMT 190 200 SQEALEWGLI DKIMQLREVP ADQ

References

[1]

"Complete sequence of plasmid 2 of Rhizobium leguminosarum bv. trifolii WSM2304." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM2304.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001193 Genomic DNA. Translation: ACI59230.1.
RefSeq	YP_002278330.1. NC_011366.1.
3D structure databases
ProteinModelPortal	B6A497.
SMR	B6A497. Positions 7-197.
ModBase	Search...
Protein-protein interaction databases
STRING	B6A497.
Protein family/group databases
MEROPS	S14.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6977446.
GenomeReviews	Gene locus Rleg2_6060 in contig CP001193_GR.
KEGG	rlt:Rleg2_6060.
PATRIC	23121254. VBIRhiLeg95088_0479.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	LYAEHCG.
ProtClustDB	PRK00277.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B6A497_RHILW

Accession

Primary (citable) accession number: B6A497

Entry history

Integrated into UniProtKB/TrEMBL:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	December 14, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)