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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Rhizobium leguminosarum bv. trifolii (strain WSM2304)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46Substrate; via amide nitrogenUniRule annotation1
Binding sitei121ATPUniRule annotation1
Binding sitei167ATPUniRule annotation1
Binding sitei194Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:Rleg2_5775
Encoded oniPlasmid pRLG2020 Publication
OrganismiRhizobium leguminosarum bv. trifolii (strain WSM2304)
Taxonomic identifieri395492 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000008330 Componenti: Plasmid pRLG202

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003838911 – 267Hydroxyethylthiazole kinaseAdd BLAST267

Structurei

3D structure databases

ProteinModelPortaliB6A3G5
SMRiB6A3G5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

B6A3G5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSRTTPGAM LTAMREKPPL VQCITNYVAM NIAANVLLAA GASPAMVHAA
60 70 80 90 100
EEADEFAGIA SALTVNIGTL STQWIDGMQA AAKATTAAGK PWVLDPVAHY
110 120 130 140 150
ATAFRRDAVA GLLALKPTII RGNASEIIAL AGGESRGQGV DSRDPVEQAE
160 170 180 190 200
GSARWLAERQ QAIVAVTGAV DFVTDGERAV RITGGSALMP QVTALGCSLT
210 220 230 240 250
CLVGAFAATA PEDLFGATVA ALATFAIAGE EAALGAAGPG SFAWRFLDAL
260
HALDAETLDA RARISAA
Length:267
Mass (Da):27,216
Last modified:November 25, 2008 - v1
Checksum:i2DD598547E1734EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001193 Genomic DNA Translation: ACI58948.1
RefSeqiWP_012555148.1, NC_011366.1

Genome annotation databases

EnsemblBacteriaiACI58948; ACI58948; Rleg2_5775
GeneIDi34189787
KEGGirlt:Rleg2_5775

Similar proteinsi

Entry informationi

Entry nameiTHIM_RHILW
AccessioniPrimary (citable) accession number: B6A3G5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: November 25, 2008
Last modified: April 25, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome
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Main funding by: National Institutes of Health