B6A1T0 (B6A1T0_RHILW) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2 HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2 HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme 2 HAMAP MF_00685
Glycogen branching enzyme 2 HAMAP MF_00685

Gene names

Name:	glgB2 HAMAP MF_00685
Ordered Locus Names:	Rleg2_5384

Encoded on

Plasmid pRLG201 EMBL ACI58564.1

Organism

Rhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP]

Taxonomic identifier

395492 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome Plasmid EMBL ACI58564.1
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	415	1	Nucleophile By similarity HAMAP MF_00685
	Active site	468	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	B6A1T0 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: D4147787C569A2D1	FASTA	737	81,862
	10 20 30 40 50 60 MNVERSELLA GIGHDALWAL IDGRHGDPFS ILGPHESGGM TIVRVYLPGA EAVDLIEAAS 70 80 90 100 110 120 GRVVTPFSIA HPAGLFAAAT GLRSGYRLKI TWPDAVQITE DPYSFGLLLG ELDLHLISEG 130 140 150 160 170 180 THYSLSRTLG AVAMSIDGIS GVRFAVWAPN ARRVSVVGDF NAWDGRRNPM RLRQSAGVWE 190 200 210 220 230 240 LFIPSLAPGE RYKFEIIDAE GTCLPQKADP VARASEAAPS TASIVASSTP FRWTDDGWMK 250 260 270 280 290 300 GRSRQARLEG AISVYEVHAG SWLRDGRDGN RYLDWVELSQ RLVPYASDMG FTHIELLPIM 310 320 330 340 350 360 EHPFGGSWGY QPLGLFAPTG RYGTPEDFAY FVDRCHGAGL GVILDWVPAH FPTDVWGLAR 370 380 390 400 410 420 FDGSALYEHE DPREGFHRDW NTLIYNLGRN EVKGFLIASA LEWLERYHVD GLRVDAVASM 430 440 450 460 470 480 LYRDYSRNEG EWIPNQYGGR ENLEAVEFFK HLNSIVHQRC PHAMTIAEES TAWPGVTKPP 490 500 510 520 530 540 EEGGLGFDIK WNMGWMHDSL SYIEKDPVYR SHHHGTMTFG MIYAYSERFM LPISHDEVVY 550 560 570 580 590 600 GKGSLLTKMP GDEWQKFANL RSYLAFMWGH PGKKLLFMGS EIAQPGEWNH DASVSWDVLD 610 620 630 640 650 660 RPQHVGIQRL VKDLNSLYGD EPALQFGDFH PQGFEWAAAD DAANSVLGML RYSEDRSSSV 670 680 690 700 710 720 LVVSNFTPVP RYGYRIGVPD DGVWIERITT DAREYGGSGL VNGALSSEPI PVHGRPVSLS 730 LTLPPLATVF LKSPSPG

References

[1]

"Complete sequence of plasmid 1 of Rhizobium leguminosarum bv. trifolii WSM2304." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM2304.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001192 Genomic DNA. Translation: ACI58564.1.
RefSeq	YP_002279304.1. NC_011368.1.
3D structure databases
ProteinModelPortal	B6A1T0.
SMR	B6A1T0. Positions 121-735.
ModBase	Search...
Protein-protein interaction databases
STRING	B6A1T0.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6978478.
GenomeReviews	Gene locus Rleg2_5384 in contig CP001192_GR.
KEGG	rlt:Rleg2_5384.
PATRIC	23123267. VBIRhiLeg95088_1485.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	RVYHQNG.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B6A1T0_RHILW

Accession

Primary (citable) accession number: B6A1T0

Entry history

Integrated into UniProtKB/TrEMBL:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	December 14, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)