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B5ZYA2 (FTHS_RHILW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Rleg2_2815
OrganismRhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP]
Taxonomic identifier395492 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length559 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 559559Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_1000196822

Regions

Nucleotide binding68 – 758ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B5ZYA2 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 86E0BDB25343DE72

FASTA55959,710
        10         20         30         40         50         60 
MPSIKSDIEI ARAAAKKPIF EIGAKLGIPV EQLVPYGHDK AKVSAEFIAG QAGKKDGKLI 

        70         80         90        100        110        120 
LVTAINPTPA GEGKTTTTVG LGDGLNRIGK KAIVCIREAS LGPCFGVKGG AAGGGFAQVV 

       130        140        150        160        170        180 
PMEDINLHFT GDFHAITSAH NLLAAMIDNH IYWGNEENID IRRITWRRVM DMNDRALRSM 

       190        200        210        220        230        240 
VSSLGGVANG FPRQGGFDIT VASEVMAILC LATDLTDLER RLGDIIIGYR FDRTPVHARD 

       250        260        270        280        290        300 
LKADGAMAVL LKDAMQPNLV QTLENNPAFV HGGPFANIAH GCNSVTATKT ALKLGDYVVT 

       310        320        330        340        350        360 
EAGFGADLGA EKFFDIKCRK AGLKPDAAVI VATVRALKMN GGVKKDDLGT EDVAALKKGC 

       370        380        390        400        410        420 
ANLGRHVANV RRFGVPVVVA INHFISDTDA EIAAVKEFVS RLGAEAILCR HWALGSAGIE 

       430        440        450        460        470        480 
ELAHKVVELA ESGQAKFQPL YGDDISLFEK IEIVASKIYH AGEVTADKAV RDQLQTWEEQ 

       490        500        510        520        530        540 
GYGKLPVCMA KTQYSFSTDP SLRGAPEGHI VTVREVRLSA GAGFVVAITG EIMTMPGLPK 

       550 
SPSAERIFLN EQGYIEGLF 

« Hide

References

[1]"Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. expand/collapse author list , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: WSM2304.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001191 Genomic DNA. Translation: ACI56085.1.
RefSeqYP_002282311.1. NC_011369.1.

3D structure databases

ProteinModelPortalB5ZYA2.
SMRB5ZYA2. Positions 7-557.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395492.Rleg2_2815.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI56085; ACI56085; Rleg2_2815.
GeneID6981559.
KEGGrlt:Rleg2_2815.
PATRIC23129772. VBIRhiLeg95088_4704.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMALKHHGGV.
OrthoDBEOG6PCPSP.

Enzyme and pathway databases

BioCycRLEG395492:GJB3-2851-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_RHILW
AccessionPrimary (citable) accession number: B5ZYA2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways