B5ZYA2 (FTHS_RHILW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Rleg2_2815

Organism

Rhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP]

Taxonomic identifier

395492 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium › Rhizobium leguminosarum › Rhizobium leguminosarum bv. trifolii

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_1000196822
Regions
	Nucleotide binding	68 – 75	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5ZYA2 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 86E0BDB25343DE72	FASTA	559	59,710
	10 20 30 40 50 60 MPSIKSDIEI ARAAAKKPIF EIGAKLGIPV EQLVPYGHDK AKVSAEFIAG QAGKKDGKLI 70 80 90 100 110 120 LVTAINPTPA GEGKTTTTVG LGDGLNRIGK KAIVCIREAS LGPCFGVKGG AAGGGFAQVV 130 140 150 160 170 180 PMEDINLHFT GDFHAITSAH NLLAAMIDNH IYWGNEENID IRRITWRRVM DMNDRALRSM 190 200 210 220 230 240 VSSLGGVANG FPRQGGFDIT VASEVMAILC LATDLTDLER RLGDIIIGYR FDRTPVHARD 250 260 270 280 290 300 LKADGAMAVL LKDAMQPNLV QTLENNPAFV HGGPFANIAH GCNSVTATKT ALKLGDYVVT 310 320 330 340 350 360 EAGFGADLGA EKFFDIKCRK AGLKPDAAVI VATVRALKMN GGVKKDDLGT EDVAALKKGC 370 380 390 400 410 420 ANLGRHVANV RRFGVPVVVA INHFISDTDA EIAAVKEFVS RLGAEAILCR HWALGSAGIE 430 440 450 460 470 480 ELAHKVVELA ESGQAKFQPL YGDDISLFEK IEIVASKIYH AGEVTADKAV RDQLQTWEEQ 490 500 510 520 530 540 GYGKLPVCMA KTQYSFSTDP SLRGAPEGHI VTVREVRLSA GAGFVVAITG EIMTMPGLPK 550 SPSAERIFLN EQGYIEGLF

References

[1]

"Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM2304.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001191 Genomic DNA. Translation: ACI56085.1.
RefSeq	YP_002282311.1. NC_011369.1.
3D structure databases
ProteinModelPortal	B5ZYA2.
SMR	B5ZYA2. Positions 7-557.
ModBase	Search...
Protein-protein interaction databases
STRING	395492.Rleg2_2815.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI56085; ACI56085; Rleg2_2815.
GeneID	6981559.
KEGG	rlt:Rleg2_2815.
PATRIC	23129772. VBIRhiLeg95088_4704.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	IIIGYNY.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	RLEG395492:GJB3-2851-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_RHILW

Accession

Primary (citable) accession number: B5ZYA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program