B5ZW73 (RNC_RHILW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	Rleg2_0998

Organism

Rhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP]

Taxonomic identifier

395492 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium › Rhizobium leguminosarum › Rhizobium leguminosarum bv. trifolii

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity. HAMAP-Rule MF_00104
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00104
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00104.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_1000094128
Regions
	Domain	12 – 137	126	RNase III
	Domain	162 – 231	70	DRBM
Sites
	Active site	54	1	Potential
	Active site	126	1	By similarity
	Metal binding	50	1	Magnesium By similarity
	Metal binding	123	1	Magnesium By similarity
	Metal binding	126	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5ZW73 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 0D413F73E1DF1495	FASTA	239	26,452
	10 20 30 40 50 60 MSKAQTLSAA DRAKLEGLIG HDFAEKERLD RALTHASART EKGGNYERLE FLGDRVLGLC 70 80 90 100 110 120 IAELLFRTFG TAGEGELSVR LNQLVSAETC AAVADELNLH LYIRTGADVK KLTGKRMMNV 130 140 150 160 170 180 RADVVESLIA AIYLDGGLEV ARRFILRYWQ GRAVRADGAK RDAKTELQEW SHAKFGVTPN 190 200 210 220 230 YRVDERSGPD HDPRFKVTVE VAGIKPETGV ERSKRAAEQV AATKMLEREG IWQQSPAGN

References

[1]

"Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM2304.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001191 Genomic DNA. Translation: ACI54292.1.
RefSeq	YP_002280518.1. NC_011369.1.
3D structure databases
ProteinModelPortal	B5ZW73.
ModBase	Search...
Protein-protein interaction databases
STRING	395492.Rleg2_0998.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI54292; ACI54292; Rleg2_0998.
GeneID	6979717.
KEGG	rlt:Rleg2_0998.
PATRIC	23125920. VBIRhiLeg95088_2802.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246809.
KO	K03685.
OMA	HENFGES.
ProtClustDB	PRK12371.
Enzyme and pathway databases
BioCyc	RLEG395492:GJB3-1008-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_RHILW

Accession

Primary (citable) accession number: B5ZW73

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program