B5ZVA2 (MASZ_RHILW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Rleg2_3986

Organism

Rhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP]

Taxonomic identifier

395492 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Malate synthase G HAMAP MF_00641		PRO_1000130896
Sites
	Active site	338	1	Proton acceptor By similarity
	Active site	629	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5ZVA2 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 421230A52CE25C56	FASTA	723	79,709
	10 20 30 40 50 60 MSRIDKNGLA IEAVLHDFLV QEVLPGLAID ADKFFADFSA IVHDLAPKNR ALLARRDELE 70 80 90 100 110 120 VKIDDWYRQH GAPADMDDYQ SFLRGIGYLL PEGSDFQVST QNVDPEIASI AGPQLVVPVM 130 140 150 160 170 180 NARYALNAAN ARWGSLYDAL YGTDAIPETD GAEKGRGYNP KRGEKVIAWV RDFLDMSVPL 190 200 210 220 230 240 QDSSWKNVGN FTVKDGILVI RSVDGEQAML VDGGHFAGYR GDAAAPTHIL LKNNGIHIKI 250 260 270 280 290 300 VIDATTAIGK TDPAHISDVW LESAITTIMD CEDSIAAVDA EDKTVVYRNW LGLMKGDLQE 310 320 330 340 350 360 EVVKGGTSFV RKLNPDLDYT GPDGTVFELH RRSLMLVRNV GHLMTNPAIL DRDGAEVPEG 370 380 390 400 410 420 ILDAVITGLI ALYDIGPAGR RKNSRTGSMY VVKPKMHGPE EVAFAVEIFS RVEDALGMAR 430 440 450 460 470 480 NTIKMGIMDE ERRTTVNLKE CIRAARERVV FINTGFLDRT GDEIHTSMEA GPMIRKGDMR 490 500 510 520 530 540 QAAWISAYEN WNVDIGLECG LAGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS 550 560 570 580 590 600 PTAATLHATH YHRVNVARVQ QGLKDRARAK LSDILSVPVA VRPNWTPEEI QRELDNNAQG 610 620 630 640 650 660 ILGYVVRWVD QGVGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHQVVT EAQIVETMKR 670 680 690 700 710 720 MAAVVDRQNE ADPLYQPMAG NFDGSIAFQA ALDLVLKGRE QPNGYTEPVL HRRRLELKAK QAG

References

[1]

"Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM2304.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001191 Genomic DNA. Translation: ACI57248.1.
RefSeq	YP_002283474.1. NC_011369.1.
3D structure databases
ProteinModelPortal	B5ZVA2.
SMR	B5ZVA2. Positions 3-722.
ModBase	Search...
Protein-protein interaction databases
STRING	B5ZVA2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6982750.
GenomeReviews	Gene locus Rleg2_3986 in contig CP001191_GR.
KEGG	rlt:Rleg2_3986.
PATRIC	23132270. VBIRhiLeg95088_5934.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG350005.
OMA	PKMHGPD.
ProtClustDB	PRK02999.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_RHILW

Accession

Primary (citable) accession number: B5ZVA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program