Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B5ZVA2 (MASZ_RHILW) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase G

EC=2.3.3.9
Gene names
Name:glcB
Ordered Locus Names:Rleg2_3986
OrganismRhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP]
Taxonomic identifier395492 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length723 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity. HAMAP-Rule MF_00641

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00641

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00641.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionTransferase
   PTMOxidation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 723723Malate synthase G HAMAP-Rule MF_00641
PRO_1000130896

Regions

Region123 – 1242Acetyl-CoA binding By similarity
Region455 – 4584Glyoxylate binding By similarity

Sites

Active site3381Proton acceptor By similarity
Active site6291Proton donor By similarity
Metal binding4301Magnesium By similarity
Metal binding4581Magnesium By similarity
Binding site1161Acetyl-CoA; via carbonyl oxygen By similarity
Binding site2741Acetyl-CoA By similarity
Binding site3111Acetyl-CoA By similarity
Binding site3381Glyoxylate By similarity
Binding site4301Glyoxylate By similarity
Binding site5391Acetyl-CoA; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue6151Cysteine sulfenic acid (-SOH) By similarity

Sequences

Sequence LengthMass (Da)Tools
B5ZVA2 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 421230A52CE25C56

FASTA72379,709
        10         20         30         40         50         60 
MSRIDKNGLA IEAVLHDFLV QEVLPGLAID ADKFFADFSA IVHDLAPKNR ALLARRDELE 

        70         80         90        100        110        120 
VKIDDWYRQH GAPADMDDYQ SFLRGIGYLL PEGSDFQVST QNVDPEIASI AGPQLVVPVM 

       130        140        150        160        170        180 
NARYALNAAN ARWGSLYDAL YGTDAIPETD GAEKGRGYNP KRGEKVIAWV RDFLDMSVPL 

       190        200        210        220        230        240 
QDSSWKNVGN FTVKDGILVI RSVDGEQAML VDGGHFAGYR GDAAAPTHIL LKNNGIHIKI 

       250        260        270        280        290        300 
VIDATTAIGK TDPAHISDVW LESAITTIMD CEDSIAAVDA EDKTVVYRNW LGLMKGDLQE 

       310        320        330        340        350        360 
EVVKGGTSFV RKLNPDLDYT GPDGTVFELH RRSLMLVRNV GHLMTNPAIL DRDGAEVPEG 

       370        380        390        400        410        420 
ILDAVITGLI ALYDIGPAGR RKNSRTGSMY VVKPKMHGPE EVAFAVEIFS RVEDALGMAR 

       430        440        450        460        470        480 
NTIKMGIMDE ERRTTVNLKE CIRAARERVV FINTGFLDRT GDEIHTSMEA GPMIRKGDMR 

       490        500        510        520        530        540 
QAAWISAYEN WNVDIGLECG LAGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS 

       550        560        570        580        590        600 
PTAATLHATH YHRVNVARVQ QGLKDRARAK LSDILSVPVA VRPNWTPEEI QRELDNNAQG 

       610        620        630        640        650        660 
ILGYVVRWVD QGVGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHQVVT EAQIVETMKR 

       670        680        690        700        710        720 
MAAVVDRQNE ADPLYQPMAG NFDGSIAFQA ALDLVLKGRE QPNGYTEPVL HRRRLELKAK 


QAG 

« Hide

References

[1]"Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. expand/collapse author list , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: WSM2304.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001191 Genomic DNA. Translation: ACI57248.1.
RefSeqYP_002283474.1. NC_011369.1.

3D structure databases

ProteinModelPortalB5ZVA2.
SMRB5ZVA2. Positions 3-722.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395492.Rleg2_3986.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI57248; ACI57248; Rleg2_3986.
GeneID6982750.
KEGGrlt:Rleg2_3986.
PATRIC23132270. VBIRhiLeg95088_5934.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2225.
HOGENOMHOG000220740.
KOK01638.
OMAPKMHGPD.
OrthoDBEOG6HJ286.
ProtClustDBPRK02999.

Enzyme and pathway databases

BioCycRLEG395492:GJB3-4042-MONOMER.
UniPathwayUPA00703; UER00720.

Family and domain databases

Gene3D2.170.170.11. 2 hits.
HAMAPMF_00641. Malate_synth_G.
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01345. malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_RHILW
AccessionPrimary (citable) accession number: B5ZVA2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways