B5ZRZ4 (LPXK_RHILW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	Rleg2_0490

Organism

Rhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP]

Taxonomic identifier

395492 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000123732
Regions
	Nucleotide binding	54 – 61	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B5ZRZ4 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 8830252D72CB1DDC	FASTA	357	38,412
	10 20 30 40 50 60 MISEAPPFWW RKADWRAWLL LPVSFLYGRI AGHRMAHGRR ASVAIPVICV GNFTVGGAGK 70 80 90 100 110 120 TPTALTIARA AKAKGLKPGF LSRGYGGSLD VTTVVDPDHH RAVAVGDEPL LLAREALTVI 130 140 150 160 170 180 SRRRVDGAQR LVAEGADLII MDDGFQSARL AIDYALLVID ATRGLGNGHI VPAGPVRAPI 190 200 210 220 230 240 RQQLRSATAL LKVGGGNAAD RIVRMAARAA KPYFTASLKV RGDNTLAGMK VLAFAGIADP 250 260 270 280 290 300 AKFFRTVESR GAEITVAKSF GDHEHLTEDE IDDILTTAER QDLLIVTTSK DFVRLSGHPG 310 320 330 340 350 KAAQLAEKCR VIEVDMVFED HLAPSLIIDR AIVACRERRL REMKAGIKAM PGKAGPL

References

[1]

"Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM2304.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001191 Genomic DNA. Translation: ACI53787.1.
RefSeq	YP_002280013.1. NC_011369.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5ZRZ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6979206.
GenomeReviews	Gene locus Rleg2_0490 in contig CP001191_GR.
KEGG	rlt:Rleg2_0490.
PATRIC	23124832. VBIRhiLeg95088_2261.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_RHILW

Accession

Primary (citable) accession number: B5ZRZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program