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B5ZRE0 (B5ZRE0_RHILW) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP-Rule MF_02002

EC=6.1.1.5 HAMAP-Rule MF_02002
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP-Rule MF_02002
Gene names
Name:ileS HAMAP-Rule MF_02002
Ordered Locus Names:Rleg2_0474 EMBL ACI53772.1
OrganismRhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP] EMBL ACI53772.1
Taxonomic identifier395492 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length963 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002 SAAS SAAS002301

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. HAMAP-Rule MF_02002

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Motif64 – 7411"HIGH" region By similarity HAMAP-Rule MF_02002
Motif637 – 6415"KMSKS" region By similarity HAMAP-Rule MF_02002

Sites

Binding site5961Aminoacyl-adenylate By similarity HAMAP-Rule MF_02002
Binding site6401ATP By similarity HAMAP-Rule MF_02002

Sequences

Sequence LengthMass (Da)Tools
B5ZRE0 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: C4204B87756DCCCE

FASTA963108,457
        10         20         30         40         50         60 
MTDTAEKIDY SKTLYLPETD FPMRAGLPQK EPELVKRWQE MGLYKKLRAS AAGREKFVLH 

        70         80         90        100        110        120 
DGPPYANGNI HIGHALNKIL KDVINRSFQM RGYDANYVPG WDCHGLPIEW KIEEKYREKG 

       130        140        150        160        170        180 
RNKDEVPVNE FRRECREFAT GWIKTQAEEF KRLGIEGDFD NPYTTMNFHA ESRIAGELLK 

       190        200        210        220        230        240 
IAASGQLYRG SKPIMWSVVE RTALAEAEVE YQDYESDTIW VKFPVVEGPA ALKDAFVVIW 

       250        260        270        280        290        300 
TTTPWTIPGN RAVSFSARVS YGLYEVTAAE NDFGPRPGEK LIFADKLAEE SFAKSKLQYK 

       310        320        330        340        350        360 
RLSDVSAADF AAMACAHPFK GLDGGYEFAV PLLDGDHVTD DAGTGFVHTA PSHGREDFDA 

       370        380        390        400        410        420 
WMSAVRTLEA RGIDSKIPFP VDDGGFYTSD APGFEGARVI DDNGKKGDAN DRVIKALIER 

       430        440        450        460        470        480 
NALFARGRLK HQYPHSWRSK KPVIFRNTPQ WFVYMDKTLA DGTTLRSRAL GAIDDTRFVP 

       490        500        510        520        530        540 
AAGQNRLRAM IEGRPDWVLS RQRAWGVPIA VFADDEGEVL VDEAVNARIL EAFEQEGADA 

       550        560        570        580        590        600 
WFADGAKERF LGNDHDHSRW KQVMDILDVW FDSGSTHTFT LEDRPDLKWP ADLYLEGSDQ 

       610        620        630        640        650        660 
HRGWFHSSLL ESAATRGRAP YNAVLTHGFT MDEKGEKMSK SKGNVTAPQE VMKDAGADIL 

       670        680        690        700        710        720 
RLWVMTSDYA DDLRVGKTII QTNVDAYRKL RNTIRWMLGT LAHDKGEEIL LADLPELEQL 

       730        740        750        760        770        780 
MLHRLAELDE LVRENYDAFD FKKIARALID FANVELSAFY FDVRKDALYC DAPSSLRRRA 

       790        800        810        820        830        840 
SLHVIRQIFD CMVTWLAPML PFTTEEAWLS RNPSAVSVHL EQFAPVAKEW RNDALAEKWK 

       850        860        870        880        890        900 
KIRAVRSVVT GALEIERKDK RIGSSLEAAP VVHIADPELM KALEGQDFTE VCITSAIEIA 

       910        920        930        940        950        960 
AGEGPAEAFR LAEVPLVSVV PKLAEGEKCA RSWRITRDVG SDPEYPDVSA RDAAALRELA 


RLA 

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References

[1]"Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. expand/collapse author list , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: WSM2304.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001191 Genomic DNA. Translation: ACI53772.1.
RefSeqYP_002279998.1. NC_011369.1.

3D structure databases

ProteinModelPortalB5ZRE0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395492.Rleg2_0474.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI53772; ACI53772; Rleg2_0474.
GeneID6979190.
KEGGrlt:Rleg2_0474.
PATRIC23124798. VBIRhiLeg95088_2244.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.
ProtClustDBPRK13804.

Enzyme and pathway databases

BioCycRLEG395492:GJB3-481-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB5ZRE0_RHILW
AccessionPrimary (citable) accession number: B5ZRE0
Entry history
Integrated into UniProtKB/TrEMBL: November 25, 2008
Last sequence update: November 25, 2008
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)