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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rhizobium leguminosarum bv. trifolii (strain WSM2304)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei596 – 5961Aminoacyl-adenylateUniRule annotation
Binding sitei640 – 6401ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciRLEG395492:GJB3-481-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.5UniRule annotationSAAS annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Rleg2_0474Imported
OrganismiRhizobium leguminosarum bv. trifolii (strain WSM2304)Imported
Taxonomic identifieri395492 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000008330 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi395492.Rleg2_0474.

Structurei

3D structure databases

ProteinModelPortaliB5ZRE0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionUniRule annotationAdd
BLAST
Motifi637 – 6415"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5ZRE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTAEKIDY SKTLYLPETD FPMRAGLPQK EPELVKRWQE MGLYKKLRAS
60 70 80 90 100
AAGREKFVLH DGPPYANGNI HIGHALNKIL KDVINRSFQM RGYDANYVPG
110 120 130 140 150
WDCHGLPIEW KIEEKYREKG RNKDEVPVNE FRRECREFAT GWIKTQAEEF
160 170 180 190 200
KRLGIEGDFD NPYTTMNFHA ESRIAGELLK IAASGQLYRG SKPIMWSVVE
210 220 230 240 250
RTALAEAEVE YQDYESDTIW VKFPVVEGPA ALKDAFVVIW TTTPWTIPGN
260 270 280 290 300
RAVSFSARVS YGLYEVTAAE NDFGPRPGEK LIFADKLAEE SFAKSKLQYK
310 320 330 340 350
RLSDVSAADF AAMACAHPFK GLDGGYEFAV PLLDGDHVTD DAGTGFVHTA
360 370 380 390 400
PSHGREDFDA WMSAVRTLEA RGIDSKIPFP VDDGGFYTSD APGFEGARVI
410 420 430 440 450
DDNGKKGDAN DRVIKALIER NALFARGRLK HQYPHSWRSK KPVIFRNTPQ
460 470 480 490 500
WFVYMDKTLA DGTTLRSRAL GAIDDTRFVP AAGQNRLRAM IEGRPDWVLS
510 520 530 540 550
RQRAWGVPIA VFADDEGEVL VDEAVNARIL EAFEQEGADA WFADGAKERF
560 570 580 590 600
LGNDHDHSRW KQVMDILDVW FDSGSTHTFT LEDRPDLKWP ADLYLEGSDQ
610 620 630 640 650
HRGWFHSSLL ESAATRGRAP YNAVLTHGFT MDEKGEKMSK SKGNVTAPQE
660 670 680 690 700
VMKDAGADIL RLWVMTSDYA DDLRVGKTII QTNVDAYRKL RNTIRWMLGT
710 720 730 740 750
LAHDKGEEIL LADLPELEQL MLHRLAELDE LVRENYDAFD FKKIARALID
760 770 780 790 800
FANVELSAFY FDVRKDALYC DAPSSLRRRA SLHVIRQIFD CMVTWLAPML
810 820 830 840 850
PFTTEEAWLS RNPSAVSVHL EQFAPVAKEW RNDALAEKWK KIRAVRSVVT
860 870 880 890 900
GALEIERKDK RIGSSLEAAP VVHIADPELM KALEGQDFTE VCITSAIEIA
910 920 930 940 950
AGEGPAEAFR LAEVPLVSVV PKLAEGEKCA RSWRITRDVG SDPEYPDVSA
960
RDAAALRELA RLA
Length:963
Mass (Da):108,457
Last modified:November 25, 2008 - v1
Checksum:iC4204B87756DCCCE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001191 Genomic DNA. Translation: ACI53772.1.
RefSeqiWP_012556716.1. NC_011369.1.
YP_002279998.1. NC_011369.1.

Genome annotation databases

EnsemblBacteriaiACI53772; ACI53772; Rleg2_0474.
KEGGirlt:Rleg2_0474.
PATRICi23124798. VBIRhiLeg95088_2244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001191 Genomic DNA. Translation: ACI53772.1.
RefSeqiWP_012556716.1. NC_011369.1.
YP_002279998.1. NC_011369.1.

3D structure databases

ProteinModelPortaliB5ZRE0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi395492.Rleg2_0474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI53772; ACI53772; Rleg2_0474.
KEGGirlt:Rleg2_0474.
PATRICi23124798. VBIRhiLeg95088_2244.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciRLEG395492:GJB3-481-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R.
    , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W.
    Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WSM2304Imported.

Entry informationi

Entry nameiB5ZRE0_RHILW
AccessioniPrimary (citable) accession number: B5ZRE0
Entry historyi
Integrated into UniProtKB/TrEMBL: November 25, 2008
Last sequence update: November 25, 2008
Last modified: April 1, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.