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B5ZRE0

- B5ZRE0_RHILW

UniProt

B5ZRE0 - B5ZRE0_RHILW

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Rleg2_0474
Organism
Rhizobium leguminosarum bv. trifolii (strain WSM2304)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei596 – 5961Aminoacyl-adenylate By similarityUniRule annotation
Binding sitei640 – 6401ATP By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationsImported, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciRLEG395492:GJB3-481-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Rleg2_0474Imported
OrganismiRhizobium leguminosarum bv. trifolii (strain WSM2304)Imported
Taxonomic identifieri395492 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
ProteomesiUP000008330: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi395492.Rleg2_0474.

Structurei

3D structure databases

ProteinModelPortaliB5ZRE0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" region By similarityUniRule annotation
Add
BLAST
Motifi637 – 6415"KMSKS" region By similarityUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5ZRE0-1 [UniParc]FASTAAdd to Basket

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MTDTAEKIDY SKTLYLPETD FPMRAGLPQK EPELVKRWQE MGLYKKLRAS    50
AAGREKFVLH DGPPYANGNI HIGHALNKIL KDVINRSFQM RGYDANYVPG 100
WDCHGLPIEW KIEEKYREKG RNKDEVPVNE FRRECREFAT GWIKTQAEEF 150
KRLGIEGDFD NPYTTMNFHA ESRIAGELLK IAASGQLYRG SKPIMWSVVE 200
RTALAEAEVE YQDYESDTIW VKFPVVEGPA ALKDAFVVIW TTTPWTIPGN 250
RAVSFSARVS YGLYEVTAAE NDFGPRPGEK LIFADKLAEE SFAKSKLQYK 300
RLSDVSAADF AAMACAHPFK GLDGGYEFAV PLLDGDHVTD DAGTGFVHTA 350
PSHGREDFDA WMSAVRTLEA RGIDSKIPFP VDDGGFYTSD APGFEGARVI 400
DDNGKKGDAN DRVIKALIER NALFARGRLK HQYPHSWRSK KPVIFRNTPQ 450
WFVYMDKTLA DGTTLRSRAL GAIDDTRFVP AAGQNRLRAM IEGRPDWVLS 500
RQRAWGVPIA VFADDEGEVL VDEAVNARIL EAFEQEGADA WFADGAKERF 550
LGNDHDHSRW KQVMDILDVW FDSGSTHTFT LEDRPDLKWP ADLYLEGSDQ 600
HRGWFHSSLL ESAATRGRAP YNAVLTHGFT MDEKGEKMSK SKGNVTAPQE 650
VMKDAGADIL RLWVMTSDYA DDLRVGKTII QTNVDAYRKL RNTIRWMLGT 700
LAHDKGEEIL LADLPELEQL MLHRLAELDE LVRENYDAFD FKKIARALID 750
FANVELSAFY FDVRKDALYC DAPSSLRRRA SLHVIRQIFD CMVTWLAPML 800
PFTTEEAWLS RNPSAVSVHL EQFAPVAKEW RNDALAEKWK KIRAVRSVVT 850
GALEIERKDK RIGSSLEAAP VVHIADPELM KALEGQDFTE VCITSAIEIA 900
AGEGPAEAFR LAEVPLVSVV PKLAEGEKCA RSWRITRDVG SDPEYPDVSA 950
RDAAALRELA RLA 963
Length:963
Mass (Da):108,457
Last modified:November 25, 2008 - v1
Checksum:iC4204B87756DCCCE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001191 Genomic DNA. Translation: ACI53772.1.
RefSeqiWP_012556716.1. NC_011369.1.
YP_002279998.1. NC_011369.1.

Genome annotation databases

EnsemblBacteriaiACI53772; ACI53772; Rleg2_0474.
GeneIDi6979190.
KEGGirlt:Rleg2_0474.
PATRICi23124798. VBIRhiLeg95088_2244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001191 Genomic DNA. Translation: ACI53772.1 .
RefSeqi WP_012556716.1. NC_011369.1.
YP_002279998.1. NC_011369.1.

3D structure databases

ProteinModelPortali B5ZRE0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395492.Rleg2_0474.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACI53772 ; ACI53772 ; Rleg2_0474 .
GeneIDi 6979190.
KEGGi rlt:Rleg2_0474.
PATRICi 23124798. VBIRhiLeg95088_2244.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RLEG395492:GJB3-481-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R.
    , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W.
    Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WSM2304.

Entry informationi

Entry nameiB5ZRE0_RHILW
AccessioniPrimary (citable) accession number: B5ZRE0
Entry historyi
Integrated into UniProtKB/TrEMBL: November 25, 2008
Last sequence update: November 25, 2008
Last modified: September 3, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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