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B5ZRE0

- B5ZRE0_RHILW

UniProt

B5ZRE0 - B5ZRE0_RHILW

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rhizobium leguminosarum bv. trifolii (strain WSM2304)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei596 – 5961Aminoacyl-adenylateUniRule annotation
    Binding sitei640 – 6401ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRLEG395492:GJB3-481-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Rleg2_0474Imported
    OrganismiRhizobium leguminosarum bv. trifolii (strain WSM2304)Imported
    Taxonomic identifieri395492 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
    ProteomesiUP000008330: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi395492.Rleg2_0474.

    Structurei

    3D structure databases

    ProteinModelPortaliB5ZRE0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi64 – 7411"HIGH" regionUniRule annotationAdd
    BLAST
    Motifi637 – 6415"KMSKS" regionUniRule annotation

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B5ZRE0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDTAEKIDY SKTLYLPETD FPMRAGLPQK EPELVKRWQE MGLYKKLRAS    50
    AAGREKFVLH DGPPYANGNI HIGHALNKIL KDVINRSFQM RGYDANYVPG 100
    WDCHGLPIEW KIEEKYREKG RNKDEVPVNE FRRECREFAT GWIKTQAEEF 150
    KRLGIEGDFD NPYTTMNFHA ESRIAGELLK IAASGQLYRG SKPIMWSVVE 200
    RTALAEAEVE YQDYESDTIW VKFPVVEGPA ALKDAFVVIW TTTPWTIPGN 250
    RAVSFSARVS YGLYEVTAAE NDFGPRPGEK LIFADKLAEE SFAKSKLQYK 300
    RLSDVSAADF AAMACAHPFK GLDGGYEFAV PLLDGDHVTD DAGTGFVHTA 350
    PSHGREDFDA WMSAVRTLEA RGIDSKIPFP VDDGGFYTSD APGFEGARVI 400
    DDNGKKGDAN DRVIKALIER NALFARGRLK HQYPHSWRSK KPVIFRNTPQ 450
    WFVYMDKTLA DGTTLRSRAL GAIDDTRFVP AAGQNRLRAM IEGRPDWVLS 500
    RQRAWGVPIA VFADDEGEVL VDEAVNARIL EAFEQEGADA WFADGAKERF 550
    LGNDHDHSRW KQVMDILDVW FDSGSTHTFT LEDRPDLKWP ADLYLEGSDQ 600
    HRGWFHSSLL ESAATRGRAP YNAVLTHGFT MDEKGEKMSK SKGNVTAPQE 650
    VMKDAGADIL RLWVMTSDYA DDLRVGKTII QTNVDAYRKL RNTIRWMLGT 700
    LAHDKGEEIL LADLPELEQL MLHRLAELDE LVRENYDAFD FKKIARALID 750
    FANVELSAFY FDVRKDALYC DAPSSLRRRA SLHVIRQIFD CMVTWLAPML 800
    PFTTEEAWLS RNPSAVSVHL EQFAPVAKEW RNDALAEKWK KIRAVRSVVT 850
    GALEIERKDK RIGSSLEAAP VVHIADPELM KALEGQDFTE VCITSAIEIA 900
    AGEGPAEAFR LAEVPLVSVV PKLAEGEKCA RSWRITRDVG SDPEYPDVSA 950
    RDAAALRELA RLA 963
    Length:963
    Mass (Da):108,457
    Last modified:November 25, 2008 - v1
    Checksum:iC4204B87756DCCCE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001191 Genomic DNA. Translation: ACI53772.1.
    RefSeqiWP_012556716.1. NC_011369.1.
    YP_002279998.1. NC_011369.1.

    Genome annotation databases

    EnsemblBacteriaiACI53772; ACI53772; Rleg2_0474.
    GeneIDi6979190.
    KEGGirlt:Rleg2_0474.
    PATRICi23124798. VBIRhiLeg95088_2244.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001191 Genomic DNA. Translation: ACI53772.1 .
    RefSeqi WP_012556716.1. NC_011369.1.
    YP_002279998.1. NC_011369.1.

    3D structure databases

    ProteinModelPortali B5ZRE0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 395492.Rleg2_0474.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACI53772 ; ACI53772 ; Rleg2_0474 .
    GeneIDi 6979190.
    KEGGi rlt:Rleg2_0474.
    PATRICi 23124798. VBIRhiLeg95088_2244.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci RLEG395492:GJB3-481-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R.
      , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W.
      Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WSM2304Imported.

    Entry informationi

    Entry nameiB5ZRE0_RHILW
    AccessioniPrimary (citable) accession number: B5ZRE0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 25, 2008
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3