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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Rhizobium leguminosarum bv. trifolii (strain WSM2304)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei256ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Rleg2_3419
OrganismiRhizobium leguminosarum bv. trifolii (strain WSM2304)
Taxonomic identifieri395492 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000008330 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003677421 – 484Glutamate--tRNA ligaseAdd BLAST484

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi395492.Rleg2_3419

Structurei

3D structure databases

ProteinModelPortaliB5ZQW4
SMRiB5ZQW4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionUniRule annotationAdd BLAST11
Motifi253 – 257"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiAYYAFDT

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B5ZQW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSGVRVRI APSPTGEPHV GTAYIALFNY LFAKKHGGEF ILRIEDTDAT
60 70 80 90 100
RSTPEFETKV LDALKWCGLE WKEGPDIGGP YGPYRQSDRK PMYQPYAEEL
110 120 130 140 150
LDKGHAFRCF CTPARLEQMR EAQRAAGKPP KYDGLCLSLT AEEVTSRMAA
160 170 180 190 200
GETTVIRMKI PAEGSCDFTD GVYGDVSIPW DSVDMQVLIK ADGMPTYHMA
210 220 230 240 250
NVIDDHLMKI THVARGEEWL ASVPKHILLY RYFGWDQPVF MHLSLMRNAD
260 270 280 290 300
KSKLSKRKNP TSISYYSALG YIPEALMNFL GLFFIQIAEG EELLTMEELS
310 320 330 340 350
AKFDPENLSK AGAIFDIQKL DWLNGRWIRE KLSEEEFQAR VLAWAMENDR
360 370 380 390 400
LKAGLRLSQT RISKLGELPD LAGFLLKSDL GLQPSDFAKI KSPPEEILEI
410 420 430 440 450
LNTVQPDLEK ILEWNVETIE AELRAIADRM GKKLKVVVSP LFVAVSGSSR
460 470 480
SLPLFDSMAI LGRSVVRQRL KLAAQAVAAL VGSK
Length:484
Mass (Da):54,238
Last modified:November 25, 2008 - v1
Checksum:i1B406A4C8FD7C73C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001191 Genomic DNA Translation: ACI56686.1
RefSeqiWP_012559001.1, NC_011369.1

Genome annotation databases

EnsemblBacteriaiACI56686; ACI56686; Rleg2_3419
GeneIDi34195052
KEGGirlt:Rleg2_3419

Similar proteinsi

Entry informationi

Entry nameiSYE_RHILW
AccessioniPrimary (citable) accession number: B5ZQW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: April 25, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health