B5ZN93 (LPXA_RHILW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Short name=UDP-N-acetylglucosamine acyltransferase
EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	Rleg2_1593

Organism

Rhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP]

Taxonomic identifier

395492 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00387
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetylglucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine. HAMAP MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP MF_00387
Subunit structure	Homotrimer By similarity. HAMAP MF_00387
Subcellular location	Cytoplasm By similarity HAMAP MF_00387.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP MF_00387		PRO_1000122724

Sequences

	Sequence		Length	Mass (Da)
	B5ZN93 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 15DC62A1C72B9D2B	FASTA	272	28,497
	10 20 30 40 50 60 MSTIAESARI HPMAVVEDGA VIGEGVKIGP FCHVGPHVVL HENVELLSHA VVAGRTVIGK 70 80 90 100 110 120 GTRIFPMAVI GGDPQSVHHG GEETTLSVGA NCTMREGVTM NTGTADFGGQ TIVGDNNLFL 130 140 150 160 170 180 ANSHVAHDCK VGNHVIMSNN VMLAGHVVIE DRVILGGGSA VHQFTRVGRQ AFVGGLSAVS 190 200 210 220 230 240 YDVIPYGMLN GNPGLLSGLN VVGMTRAGVD RAVIHRVRRA YKSIFEGTGS VRENAAAIRD 250 260 270 EYADCEQAVQ ILDFIAADSD RALSSPTRGQ KG

References

[1]

"Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM2304.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001191 Genomic DNA. Translation: ACI54883.1.
RefSeq	YP_002281109.1. NC_011369.1.
3D structure databases
ProteinModelPortal	B5ZN93.
ModBase	Search...
Protein-protein interaction databases
STRING	B5ZN93.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6980329.
GenomeReviews	Gene locus Rleg2_1593 in contig CP001191_GR.
KEGG	rlt:Rleg2_1593.
PATRIC	23127178. VBIRhiLeg95088_3414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG659295.
OMA	REFCTFN.
ProtClustDB	PRK05289.
Family and domain databases
HAMAP	MF_00387. LpxA. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR010137. Lipid_A_lpxA. IPR011004. Trimer_LpxA-like. [Graphical view]
KO	K00677.
Pfam	PF00132. Hexapep. 4 hits. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01852. Lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_RHILW

Accession

Primary (citable) accession number: B5ZN93

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program