B5ZN91 (B5ZN91_RHILW) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523
EC=2.3.1.- HAMAP MF_00523

Gene names

Name:	lpxD HAMAP MF_00523
Ordered Locus Names:	Rleg2_1591

Organism

Rhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP]

Taxonomic identifier

395492 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523 UDP-3-O-(3-hydroxytetradecanoyl)glucosamine + (R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] = UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + [acyl-carrier-protein]. SAAS SAAS020573
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523 Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 3/6. SAAS SAAS020573
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily. HAMAP MF_00523

Ontologies

Keywords
Biological process	Lipid A biosynthesis HAMAP MF_00523 SAAS SAAS020573 Lipid synthesis
Domain	Repeat HAMAP MF_00523 SAAS SAAS020573
Molecular function	Acyltransferase HAMAP MF_00523 SAAS SAAS020573 EMBL ACI54881.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B5ZN91 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 7D463F33FAA82190	FASTA	354	36,612
	10 20 30 40 50 60 MEQNVFFLPH EGLKLAELAE FLGAELANSD HADIVVRSVA PINRARAGDV CYILSRRNRD 70 80 90 100 110 120 ELATCEASAV ICDKALADLV PPHIPIILSS NPHAAFAMAG GLFYPAALRP VVFSGEGEIA 130 140 150 160 170 180 PSAVIDPSAR LEKGVIVEPL AVIGAHAEIG EGTRIGAHSL IGPGVKIGRD CSIAAGASIL 190 200 210 220 230 240 CALIGNGVII HNGVRIGQDG FGYAPGPRGM IKIVQIGRVI IQDKVEIGAN TTIDRGAMDD 250 260 270 280 290 300 TVIGEGTKID NQVQIGHNVQ IGRHCAIVAQ VGIAGSTKIG NGVQIGGQVG IKGHVTIGDG 310 320 330 340 350 VQIAAKSGIM TDLAAGGQYG GVPARPLKDY LREAAQQVSK SKLRGRNPGG KEND

References

[1]

"Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM2304.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001191 Genomic DNA. Translation: ACI54881.1.
RefSeq	YP_002281107.1. NC_011369.1.
3D structure databases
ProteinModelPortal	B5ZN91.
ModBase	Search...
Protein-protein interaction databases
STRING	B5ZN91.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6980327.
GenomeReviews	Gene locus Rleg2_1591 in contig CP001191_GR.
KEGG	rlt:Rleg2_1591.
PATRIC	23127174. VBIRhiLeg95088_3412.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	VQIGRVI.
ProtClustDB	PRK00892.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 3 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B5ZN91_RHILW

Accession

Primary (citable) accession number: B5ZN91

Entry history

Integrated into UniProtKB/TrEMBL:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)