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Protein

Urease subunit alpha

Gene

ureC

Organism
Rhizobium leguminosarum bv. trifolii (strain WSM2304)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit alpha (ureC), Urease subunit beta (ureB), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi136Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi138Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi219Nickel 1; via carbamate groupUniRule annotation1
Metal bindingi219Nickel 2; via carbamate groupUniRule annotation1
Binding sitei221SubstrateUniRule annotation1
Metal bindingi248Nickel 2; via pros nitrogenUniRule annotation1
Metal bindingi274Nickel 2; via tele nitrogenUniRule annotation1
Active sitei322Proton donorUniRule annotation1
Metal bindingi362Nickel 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:Rleg2_3051
OrganismiRhizobium leguminosarum bv. trifolii (strain WSM2304)
Taxonomic identifieri395492 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000008330 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001888901 – 570Urease subunit alphaAdd BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Proteomic databases

PRIDEiB5ZMP0

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi395492.Rleg2_3051

Structurei

3D structure databases

ProteinModelPortaliB5ZMP0
SMRiB5ZMP0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 570UreaseUniRule annotationAdd BLAST440

Sequence similaritiesi

Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM Bacteria
COG0804 LUCA
HOGENOMiHOG000075064
KOiK01428
OMAiGFDSHIH

Family and domain databases

CDDicd00375 Urease_alpha, 1 hit
Gene3Di2.30.40.10, 2 hits
HAMAPiMF_01953 Urease_alpha, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
IPR011612 Urease_alpha_N_dom
IPR017950 Urease_AS
IPR005848 Urease_asu
IPR017951 Urease_asu_c
IPR029754 Urease_Ni-bd
PANTHERiPTHR43440 PTHR43440, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
PF00449 Urease_alpha, 1 hit
PRINTSiPR01752 UREASE
SUPFAMiSSF51338 SSF51338, 2 hits
SSF51556 SSF51556, 2 hits
TIGRFAMsiTIGR01792 urease_alph, 1 hit
PROSITEiView protein in PROSITE
PS01120 UREASE_1, 1 hit
PS00145 UREASE_2, 1 hit
PS51368 UREASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

B5ZMP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYKISRAAY AGMFGPTTGD KVRLADTELF IEIEKDFTTY GEEVKFGGGK
60 70 80 90 100
VIRDGMGQSQ VTRADGAVDT VITNAVIVDH SGVYKADIGL KDGRIVAIGK
110 120 130 140 150
AGNPDMQPGV NIIVGPGTEA IAAEGKIVTA GGMDSHIHFI APQQIEEALM
160 170 180 190 200
SGMTCMLGGG TGPAHGTLAT TCTPGPWHLA RMIEAADAFP MNLAFAGKGN
210 220 230 240 250
ASLPGALTEM VLAGATSLKL HEDWGTTPGA IDCCLSVADE YDVQVMIHTD
260 270 280 290 300
TLNESGFVED TIGAIKGRTI HAFHTEGAGG GHAPDIIKIC GQPNVIPSST
310 320 330 340 350
NPTRPYTVNT IAEHLDMLMV CHHLSPSIPE DIAFAESRIR KETIAAEDIL
360 370 380 390 400
HDIGAFSIIS SDSQAMGRVG EVAIRTWQTA DKMKRQRGRL KQENGDNDNF
410 420 430 440 450
RVRRYIAKYT INPAIAHGLS HEIGSVEVGK RADLVLWNPA FFGVKPDMVL
460 470 480 490 500
LGGSIAAAPM GDPNASIPTP QPVHYRPMFA SYGRSLTNSS VTFVSQASLD
510 520 530 540 550
AGLKGRLGVA KQLVAVKNTR GGISKASMIH NDLTPEIEVD PETYEVRANG
560 570
ELLTCEPATV LPMAQRYFLF
Length:570
Mass (Da):60,688
Last modified:November 25, 2008 - v1
Checksum:iA73D55623A5D25A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001191 Genomic DNA Translation: ACI56318.1
RefSeqiWP_012558726.1, NC_011369.1

Genome annotation databases

EnsemblBacteriaiACI56318; ACI56318; Rleg2_3051
GeneIDi34194774
KEGGirlt:Rleg2_3051

Similar proteinsi

Entry informationi

Entry nameiURE1_RHILW
AccessioniPrimary (citable) accession number: B5ZMP0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 25, 2008
Last modified: May 23, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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