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B5ZME5 (B5ZME5_RHILW) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Valine--tRNA ligase HAMAP-Rule MF_02004
EC=6.1.1.9 HAMAP-Rule MF_02004
Alternative name(s):
Valyl-tRNA synthetase HAMAP-Rule MF_02004
Gene names
Name:valS HAMAP-Rule MF_02004
Ordered Locus Names:Rleg2_1475 EMBL ACI54767.1
OrganismRhizobium leguminosarum bv. trifolii (strain WSM2304) [Complete proteome] [HAMAP] EMBL ACI54767.1
Taxonomic identifier395492 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium ›

Protein attributes

Sequence length947 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Catalytic activity

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP-Rule MF_02004 SAAS SAAS019499

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02004 SAAS SAAS019499.

Domain

The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity. HAMAP-Rule MF_02004

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP-Rule MF_02004

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. HAMAP-Rule MF_02004

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Coiled coil875 – 94672 By similarity HAMAP-Rule MF_02004
Motif45 – 5511"HIGH" region By similarity HAMAP-Rule MF_02004
Motif591 – 5955"KMSKS" region By similarity HAMAP-Rule MF_02004

Sites

Binding site5941ATP By similarity HAMAP-Rule MF_02004

Sequences

Sequence LengthMass (Da)Tools
B5ZME5 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 31C9368DA4542A4B

FASTA947107,129
        10         20         30         40         50         60 
MLDKTYDSAA VEPKIAAKWD EEDAFRAGAN AKPGAETFTI VIPPPNVTGS LHMGHALNNT 

        70         80         90        100        110        120 
LQDIMVRFER MRGKDVLWQP GMDHAGIATQ MVVERKLMEQ QLPGRREMGR EAFIDKIWEW 

       130        140        150        160        170        180 
KAESGGLIFN QLKRLGASCD WSRERFTMDE GLSQAVLEVF VTLYKEGLIY KDKRLVNWDP 

       190        200        210        220        230        240 
KLLTAISDME VEQHEMKGHL WHLRYPLEPG VTYQYPIAFD EEGKPTEFET RDYIVVATTR 

       250        260        270        280        290        300 
PETMLGDTGV AVNPEDERYK PIVGKHVILP IVGRKIPIVA DDYADPTAGT GAVKITPAHD 

       310        320        330        340        350        360 
FNDFEVGKRA KLRAINVMNV DATITIKENE DFLEGLDNPA ALHGAWDRLE GQDRFYARKV 

       370        380        390        400        410        420 
IVEIFEEAGL LDKIEPHKNM VPHGDRGGVP IEPRLTEQWF VDNKTLGQPA LESVREGKTR 

       430        440        450        460        470        480 
FIPRNWENTY FNWLENIEPW CISRQLWWGH QIPAWYGPDG QVFVEKTEEE ALQAAIQHYL 

       490        500        510        520        530        540 
SHEGPMKAYV EDLLENFKPG EILTRDEDVL DTWFSSALWP FSTLGWPDQT PELARYYPTS 

       550        560        570        580        590        600 
VLVTGFDIIP FWVVRMMQMG LHFMKDENGD PVEPFHTIYI HALVRDKNGQ KMSKSKGNVI 

       610        620        630        640        650        660 
DPLELIDEYG ADALRFTLAI MAAQGRDVKL DPARIAGYRN FGTKLWNATR FAEMNGAKSD 

       670        680        690        700        710        720 
PHFVPEAAEL TINRWILTEL ARTERDVTEA LEAFRFNDAA GALYRFVWNE VCDWYLELLK 

       730        740        750        760        770        780 
PVFNGEDEGA KAEAQACSAY ILEEIYKLLH PFMPFMTEEL WAHTAGEGKE RDTLVCHAEW 

       790        800        810        820        830        840 
PSPSYADNGA ADEINWLIDL VSGIRSVRAE MNVPPSATAP LVVVKANNLT RERLFRHDAA 

       850        860        870        880        890        900 
IKRLARVEAI SQADDAPKGA AQIVVDEATI CLPLGNLIDL STEKARLEKA IGKMEGEISR 

       910        920        930        940 
IDGKLSNEKF VANANPEVVE AERERLEELK GQIASLKTAL SRVSEAG

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References

[1]"Complete sequence of chromosome of Rhizobium leguminosarum bv. trifolii WSM2304."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Yates R. expand/collapse author list , Ardley J., Tiwari R.P., O'Hara G., Howieson J., Brau L., Reeve W.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: WSM2304 EMBL ACI54767.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001191 Genomic DNA. Translation: ACI54767.1.
RefSeqYP_002280993.1. NC_011369.1.

3D structure databases

ProteinModelPortalB5ZME5.
ModBaseSearch...

Protein-protein interaction databases

STRING395492.Rleg2_1475.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI54767; ACI54767; Rleg2_1475.
GeneID6980205.
KEGGrlt:Rleg2_1475.
PATRIC23126920. VBIRhiLeg95088_3291.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0525.
HOGENOMHOG000020093.
KOK01873.
OMAIMDALIR.
ProtClustDBPRK05729.

Enzyme and pathway databases

BioCycRLEG395492:GJB3-1496-MONOMER.

Family and domain databases

Gene3D1.10.287.380. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02004. Val_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERPTHR11946:SF5. PTHR11946:SF5. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSPR00986. TRNASYNTHVAL.
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00422. valS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB5ZME5_RHILW
AccessionPrimary (citable) accession number: B5ZME5
Entry history
Integrated into UniProtKB/TrEMBL: November 25, 2008
Last sequence update: November 25, 2008
Last modified: May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info