B5ZLF7 (B5ZLF7_GLUDA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121
Short name=Asp/Glu-ADT subunit B HAMAP-Rule MF_00121
EC=6.3.5.- HAMAP-Rule MF_00121

Gene names

Name:	gatB HAMAP-Rule MF_00121
Ordered Locus Names:	Gdia_3373 EMBL ACI53099.1

Organism

Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5) [Complete proteome] [HAMAP] EMBL ACI53099.1

Taxonomic identifier

272568 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconacetobacter › Gluconacetobacter diazotrophicus

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. SAAS SAAS003789 HAMAP-Rule MF_00121
Catalytic activity	ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. SAAS SAAS003789 HAMAP-Rule MF_00121 ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity. SAAS SAAS003789 HAMAP-Rule MF_00121
Sequence similarities	Belongs to the gatB/gatE family. GatB subfamily. HAMAP-Rule MF_00121

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00121
Ligand	ATP-binding HAMAP-Rule MF_00121 Nucleotide-binding
Molecular function	Ligase HAMAP-Rule MF_00121 Transferase EMBL ACI53099.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B5ZLF7 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: C526819E6F0D9A49	FASTA	482	52,907
	10 20 30 40 50 60 MTYLVEGRTG PWELVVGLEV HAQVISRSKL FSGASASYGG EPNTHVSLVD AGFPGMLPVI 70 80 90 100 110 120 NRECVAQAVR TGLGLKARIN LESRFDRKNY FYADLPTGYQ ISQFAHPIIG EGAVEIELAD 130 140 150 160 170 180 GATRTIGITR LHLEQDAGKS MHDQDPTRSF IDLNRAGVAL MEIVSEPDIR TPEEAGAYLR 190 200 210 220 230 240 KLRMILRYLG TCDGNMEEGS MRADVNVSVR KAGEPFRTRC EIKNVNSIRY VMQAIEVEAV 250 260 270 280 290 300 RQVEIWESGG EVDQETRLFD PARGETRSLR SKEDAHDYRY FPDPDLLPLV IEEAWVESLA 310 320 330 340 350 360 AVLPELPDAK RQRFQDEYGI PRYDAGVLVA EQATADFYET VARGRDPRLA ANWVIGDLFA 370 380 390 400 410 420 ALNRTGRTIQ DSPISADALG GMLDLVTDGT INGKIAKEVF EDMLETGDPA AAIVERKGLR 430 440 450 460 470 480 QVTDTGAIDA AVQAVLAANA DKLAEYRSGK DKLFGFFVGQ VMKAMAGKGN PALVNEALKK VL

References

[1]

"Complete sequence of chromosome of Gluconacetobacter diazotrophicus PAl 5." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Triplett E.W. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49037 / DSM 5601 / PAl5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001189 Genomic DNA. Translation: ACI53099.1.
RefSeq	YP_002277714.1. NC_011365.1.
3D structure databases
ProteinModelPortal	B5ZLF7.
ModBase	Search...
Protein-protein interaction databases
STRING	272568.GDI_2977.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI53099; ACI53099; Gdia_3373.
GeneID	6976818.
KEGG	gdj:Gdia_3373.
PATRIC	22055180. VBIGluDia203729_3353.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0064.
HOGENOM	HOG000223743.
KO	K02434.
OMA	KNYFYAD.
ProtClustDB	PRK05477.
Family and domain databases
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B5ZLF7_GLUDA

Accession

Primary (citable) accession number: B5ZLF7

Entry history

Integrated into UniProtKB/TrEMBL:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	May 29, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)