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B5ZCB4 (SYE_UREU1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:UUR10_0704
OrganismUreaplasma urealyticum serovar 10 (strain ATCC 33699 / Western) [Complete proteome] [HAMAP]
Taxonomic identifier565575 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeUreaplasma

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 482482Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_1000090119

Regions

Motif9 – 1911"HIGH" region HAMAP-Rule MF_00022
Motif252 – 2565"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2551ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B5ZCB4 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: E699C2AD35A1973C

FASTA48255,593
        10         20         30         40         50         60 
MKIRTRYAPS PTGYLHIGGA RTALFNYLLA KAYDGDFIIR IEDTDVERNV EGGIDSQFNF 

        70         80         90        100        110        120 
LEWMGIVADE SIRNPKTFGP YIQSQKLKHY EALALDLVAQ KKAYFCFCSK ERLDADRELA 

       130        140        150        160        170        180 
EKLHETPKYK RHCLNLNEQT IQANLLANKE YTIRLKIDEN NEYSWDDLIR GKISIPGSAL 

       190        200        210        220        230        240 
TDPVILKSNK IAMYNFAVVI DDYEMQISHV IRGEEHISNT PYQLAIAQAL NYDLSKIKYG 

       250        260        270        280        290        300 
HLSIIVDETG KKLSKRNLAL KQFVSDYEKD GYWPHAITNF VALLGWSPKN NQEIMSLVEM 

       310        320        330        340        350        360 
VENFDVNNLS KSPAFFDINK MNWFSTQYFN NISQDEFIDF VKTHPLTKEL VLKDTTFIDK 

       370        380        390        400        410        420 
ALLFKSHIVN LKQLINLVDE QFNSNKQLLE EDVNHIKNNQ LTNVVQVFYE QLIVSEKFDE 

       430        440        450        460        470        480 
QSIKEVIKQV QKTTNNKGAN LYMPIRIATT FSSHGPELAK TIYYLGRENV LKNLQSILKV 


LG 

« Hide

References

[1]"Genome sequence of Ureaplasma urealyticum serovar 10 ATCC-33699."
Shrivastava S., Methe B.A., Glass J., White K., Duffy L.B.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33699 / Western.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001184 Genomic DNA. Translation: ACI60133.1.
RefSeqYP_002285054.1. NC_011374.1.

3D structure databases

ProteinModelPortalB5ZCB4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING565575.UUR10_0704.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI60133; ACI60133; UUR10_0704.
GeneID6984189.
KEGGuue:UUR10_0704.
PATRIC20536264. VBIUreUre98799_0672.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMALMLFGRD.
OrthoDBEOG6DRPF7.
ProtClustDBPRK01406.

Enzyme and pathway databases

BioCycUURE565575:GBZS-690-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_UREU1
AccessionPrimary (citable) accession number: B5ZCB4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: February 19, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries