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B5ZB79 (TRMB_UREU1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:UUR10_0265
OrganismUreaplasma urealyticum serovar 10 (strain ATCC 33699 / Western) [Complete proteome] [HAMAP]
Taxonomic identifier565575 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeUreaplasma

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136374

Regions

Region199 – 2024Substrate binding By similarity

Sites

Active site1191 By similarity
Binding site451S-adenosyl-L-methionine By similarity
Binding site701S-adenosyl-L-methionine By similarity
Binding site971S-adenosyl-L-methionine By similarity
Binding site1191S-adenosyl-L-methionine By similarity
Binding site1231Substrate By similarity
Binding site1551Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B5ZB79 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 7D4F73EEF46E10CB

FASTA22426,258
        10         20         30         40         50         60 
MRLRNNANAP LYLKSQEQYI VNDQQLLKND LAKVFKNPHL PLHIEIGMGK GDFIVENALH 

        70         80         90        100        110        120 
NPQINYLGIE KFPTVIVKAH KKALKHKLDN LAMICFDANA ILDLLNSKSI DKIYLNFSDP 

       130        140        150        160        170        180 
WPKKRHAKKR LTNPYFLEKF AALLKDDGLI EFKTDNESLF HYTIYDVLLN DLTKYEILFL 

       190        200        210        220 
TYNLYALVNN VELLKNIPTE YEKKFVMQGQ RIKKVSFRFL KKND 

« Hide

References

[1]"Genome sequence of Ureaplasma urealyticum serovar 10 ATCC-33699."
Shrivastava S., Methe B.A., Glass J., White K., Duffy L.B.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33699 / Western.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001184 Genomic DNA. Translation: ACI60254.1.
RefSeqYP_002284669.1. NC_011374.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING565575.UUR10_0265.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI60254; ACI60254; UUR10_0265.
GeneID6984076.
KEGGuue:UUR10_0265.
PATRIC20535372. VBIUreUre98799_0257.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAGCGKGEY.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycUURE565575:GBZS-264-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_UREU1
AccessionPrimary (citable) accession number: B5ZB79
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: June 11, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways