B5ZAB7 (DXS_HELPG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	HPG27_331

Organism

Helicobacter pylori (strain G27) [Complete proteome] [HAMAP]

Taxonomic identifier

563041 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000115746

Sequences

	Sequence		Length	Mass (Da)
	B5ZAB7 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: EB6A862C0BF1B83E	FASTA	618	67,690
	10 20 30 40 50 60 MILQNRTFDL NPNDIAGLEL VCQTLRNRIL EVVSANGGHL SSSLGAVELI VGMHALFDCQ 70 80 90 100 110 120 KNPFIFDTSH QAYAHKLLTG RFESFSTLRQ FKGLSGFTKP SESAYDYFIA GHSSTSVSIG 130 140 150 160 170 180 VGVAKAFCLK QALGMPIALL GDGSISAGIF YEALNELGDR KYPMIMILND NEMSISTPIG 190 200 210 220 230 240 ALSKALSQLM KGPFYQSFRS KVKKILSTLP ESVNYLASRF EESFKLITPG VFFEELGINY 250 260 270 280 290 300 IGPINGHDLS AIIETLKLAK ELKEPVLIHA QTLKGKGYKI AEGRYEKWHG VGPFDLDTGL 310 320 330 340 350 360 SKKSKSAILS PTEAYSNTLL ELAKKDEKIV GVTAAMPSGT GLDKLIDAYP LRFFDVAIAE 370 380 390 400 410 420 QHALTSSSAM AKEGFKPFVS IYSTFLQRAY DSIVHDACIS SLPIKLAIDR AGIVGEDGET 430 440 450 460 470 480 HQGLLDVSYL RSIPNMVIFA PRDNETLKNA VRFANEHDSS PCAFRYPRGS FVLKEGVFEP 490 500 510 520 530 540 SGFVLGQSEL LKKEGEILLI GYGNGVGRAH LVQLALKEKN IECALLDLRF LKPLDPNLSA 550 560 570 580 590 600 IVAPYQKLYV FSDNYKLGGV ASAILEFLSE QNILKPVKSF EIIDEFIMHG NTALVEKSLG 610 LDTESLTDAI LKDLGQER

References

[1]

"The complete genome sequence of Helicobacter pylori strain G27." Baltrus D.A., Amieva M.R., Covacci A., Lowe T.M., Merrell D.S., Ottemann K.M., Stein M., Salama N.R., Guillemin K. J. Bacteriol. 191:447-448(2009) [PubMed: 18952803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001173 Genomic DNA. Translation: ACI27097.1.
RefSeq	YP_002265963.1. NC_011333.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B5ZAB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6963218.
GenomeReviews	Gene locus HPG27_331 in contig CP001173_GR.
KEGG	hpg:HPG27_331.
PATRIC	20598616. VBIHelPyl113476_0366.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	RFANEHD.
ProtClustDB	PRK05444.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_HELPG

Accession

Primary (citable) accession number: B5ZAB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program