B5ZA97 (NADE_HELPG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	HPG27_311

Organism

Helicobacter pylori (strain G27) [Complete proteome] [HAMAP]

Taxonomic identifier

563041 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099026
Regions
	Nucleotide binding	31 – 38	8	ATP By similarity
Sites
	Active site	33	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5ZA97 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: E80168F7DE80B294	FASTA	260	29,289
	10 20 30 40 50 60 MQKDYQKLIV YLCDFLEKEA QKRGFKKVVY GLSGGLDSAV VGVLCQKVFK ENAHALLMPS 70 80 90 100 110 120 SVSMPESKTD ALNLCETFSI PYTEYSIAPY DKIFGSHFKD ASLTRKGNFC ARLRMAFLYD 130 140 150 160 170 180 YSLKSDSLVI GTSNKSERIL GYGTLFGDLA CAINPIGELF KTEVYELAYY LNIPKKILNK 190 200 210 220 230 240 PPSADLFVGQ SDEKDLGYPY SVIDPLLKDI EALFQTKPIH LETLTQLGYD EILVKNIISR 250 260 IQKNAFKLES PTIAKRFNPK

References

[1]

"The complete genome sequence of Helicobacter pylori strain G27." Baltrus D.A., Amieva M.R., Covacci A., Lowe T.M., Merrell D.S., Ottemann K.M., Stein M., Salama N.R., Guillemin K. J. Bacteriol. 191:447-448(2009) [PubMed: 18952803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001173 Genomic DNA. Translation: ACI27077.1.
RefSeq	YP_002265943.1. NC_011333.1.
3D structure databases
ProteinModelPortal	B5ZA97.
ModBase	Search...
Protein-protein interaction databases
STRING	B5ZA97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6963197.
GenomeReviews	Gene locus HPG27_311 in contig CP001173_GR.
KEGG	hpg:HPG27_311.
PATRIC	20598572. VBIHelPyl113476_0345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	KLIVYLC.
ProtClustDB	PRK13980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_HELPG

Accession

Primary (citable) accession number: B5ZA97

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program