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B5Z943 (SYI_HELPG) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:HPG27_1345
OrganismHelicobacter pylori (strain G27) [Complete proteome] [HAMAP]
Taxonomic identifier563041 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter ›

Protein attributes

Sequence length920 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 920920Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_1000189171

Regions

Motif58 – 6811"HIGH" region HAMAP-Rule MF_02002
Motif610 – 6145"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8951Zinc By similarity
Metal binding8981Zinc By similarity
Metal binding9101Zinc By similarity
Metal binding9131Zinc By similarity
Binding site5691Aminoacyl-adenylate By similarity
Binding site6131ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B5Z943 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 6834E4171FF70987

FASTA920106,101
        10         20         30         40         50         60 
MKEYKDTLNL NTTTFSMKGN LSVNEPKTYA KWQEQQAFKR MQNRKDNHGD FTLHDGPPYA 

        70         80         90        100        110        120 
NGHLHLGHAL NKILKDIVIK REYFKGNKIY YTPGWDCHGL PIEQQILERL EKEKTSLENP 

       130        140        150        160        170        180 
TLFREKCRDH AKKFLEIQKN EFLQLGVLGD FEDPYKTMDF KFEASIYRAL VEVAKKGLLK 

       190        200        210        220        230        240 
ERHKPIYWSY ACESALAEAE VEYKMKKSPS IFVAFGLKKE SLEKLKVKKA SLVIWTTTPW 

       250        260        270        280        290        300 
TLYANVAIAL KKDALYALTQ KGYLVAKVLH EKLAALGVVD SEIAHEFNAN DLEYLKATNP 

       310        320        330        340        350        360 
LNQRDSLITL GEHVGLEDGT GAVHTAPGHG EEDYYLGLKY NLEVLMSVDE KGCYNEGIIH 

       370        380        390        400        410        420 
KKLLDESYLG EHVFKAQKRI IEQLGDSLLL EQEIEHSYPH CWRTHKPVIY RATTQWFILM 

       430        440        450        460        470        480 
DEPFIQNDGS QKTLREVALN AIEKVEFVPS SGKNRLKTMI ENRPDWCLSR QRKWGVPLAF 

       490        500        510        520        530        540 
FIDKRTNKPC FESEVLEHVA NLFEKKGCDV WWESSVKDLL PPSYQEDSKH YEKVMHILDV 

       550        560        570        580        590        600 
WFDSGSTFKA VLEDYYGEKG QSPSDVILEG SDQHRGWFQS SLLIGCVLNN QAPFKKVITH 

       610        620        630        640        650        660 
GFIVDEKGEK MSKSKGNVVS LDNLLKKHGS DVVRLWVAFN DYQNDLRVSQ TFFIQTEQHY 

       670        680        690        700        710        720 
KKFRNTLKFL LANFSDMDLK DLERSHNFSP LDHFILETLE TTSTGVNSAF EEHDFVKGLN 

       730        740        750        760        770        780 
ILMAFVTNEL SGIYLDACKD SLYCDSKNNK KRQAIQMVLL AVASQLCYFL APILTHTIEE 

       790        800        810        820        830        840 
VLEHSQVLCA FLQAKDVFDL KGINILEKLR LKEFKKPENF EAVLALRSAF NEELDRLKKE 

       850        860        870        880        890        900 
GVIKNSLECA IEVKEKALRE NLVEELLMVS FVGVAKEKIS ETPAFTLFKA PFYKCPRCWR 

       910        920 
FKSELENTPC KRCEEVLKER

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References

[1]"The complete genome sequence of Helicobacter pylori strain G27."
Baltrus D.A., Amieva M.R., Covacci A., Lowe T.M., Merrell D.S., Ottemann K.M., Stein M., Salama N.R., Guillemin K.
J. Bacteriol. 191:447-448(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: G27.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001173 Genomic DNA. Translation: ACI28092.1.
RefSeqYP_002266958.1. NC_011333.1.

3D structure databases

ProteinModelPortalB5Z943.
ModBaseSearch...

Protein-protein interaction databases

STRING563041.HPG27_1345.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI28092; ACI28092; HPG27_1345.
GeneID6964263.
KEGGhpg:HPG27_1345.
PATRIC20600860. VBIHelPyl113476_1460.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAKQVLTHG.
ProtClustDBPRK05743.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_HELPG
AccessionPrimary (citable) accession number: B5Z943
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 25, 2008
Last modified: May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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