B5Z935 (QUEF_HELPG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	HPG27_1336

Organism

Helicobacter pylori (strain G27) [Complete proteome] [HAMAP]

Taxonomic identifier

563041 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00818
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00818		PRO_1000134304

Sequences

	Sequence		Length	Mass (Da)
	B5Z935 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 43943B4102AD4E2E	FASTA	148	17,065
	10 20 30 40 50 60 MTPELNLKSL GAKTPYIFEY NSDLLEAFPN PNPNLDPLIT LECKEFTSLC PITSQPDFGV 70 80 90 100 110 120 IFIRYIPKDK MVESKSLKLY LFSYRNHGSF HESCINTILL DLVQLLEPKY LEVYGDFASR 130 140 GGIAIKPFVN YAIKEYQEFK EKRLLNAK

References

[1]

"The complete genome sequence of Helicobacter pylori strain G27." Baltrus D.A., Amieva M.R., Covacci A., Lowe T.M., Merrell D.S., Ottemann K.M., Stein M., Salama N.R., Guillemin K. J. Bacteriol. 191:447-448(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001173 Genomic DNA. Translation: ACI28084.1.
RefSeq	YP_002266950.1. NC_011333.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	563041.HPG27_1336.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI28084; ACI28084; HPG27_1336.
GeneID	6964254.
KEGG	hpg:HPG27_1336.
PATRIC	20600840. VBIHelPyl113476_1450.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HOG000009528.
KO	K09457.
OMA	LECKEFT.
ProtClustDB	PRK13258.
Enzyme and pathway databases
UniPathway	UPA00392.
Family and domain databases
HAMAP	MF_00818. QueF_type1.
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_HELPG

Accession

Primary (citable) accession number: B5Z935

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program