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B5Z928 (BIOB_HELPG) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:HPG27_1329
OrganismHelicobacter pylori (strain G27) [Complete proteome] [HAMAP]
Taxonomic identifier563041 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282Biotin synthase HAMAP-Rule MF_01694
PRO_0000381426

Sites

Metal binding171Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding211Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding241Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding961Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1541Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2211Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B5Z928 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 0F67607B7FE329B9

FASTA28231,476
        10         20         30         40         50         60 
MQEIFLCSIS NVRSGDCKED CAYCTQSSHH QGAIKRYKFK DEKVVLQEAR ALRELGALGF 

        70         80         90        100        110        120 
CLVTSGRELD DEKCEYIAKL AKAINKEELG LHLIACCGRA DLEQLEFLRD AGIHSYNHNL 

       130        140        150        160        170        180 
ETSQNFFPKI CSTHTWEERF ITCENALRAG LGLCSGGIFG LNESWEDRIE MLRALASLSP 

       190        200        210        220        230        240 
HTTPINFFIK NPVLPIDAET LSADEALECV LLAKEFLPNA RLMVAGGREV VFKDNDKKEA 

       250        260        270        280 
KLFEYGINAV VLGDYLTTKG KAPKKDIEKL LSYGLTMATS CH 

« Hide

References

[1]"The complete genome sequence of Helicobacter pylori strain G27."
Baltrus D.A., Amieva M.R., Covacci A., Lowe T.M., Merrell D.S., Ottemann K.M., Stein M., Salama N.R., Guillemin K.
J. Bacteriol. 191:447-448(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: G27.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001173 Genomic DNA. Translation: ACI28077.1.
RefSeqYP_002266943.1. NC_011333.1.

3D structure databases

ProteinModelPortalB5Z928.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING563041.HPG27_1329.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI28077; ACI28077; HPG27_1329.
GeneID6964245.
KEGGhpg:HPG27_1329.
PATRIC20600818. VBIHelPyl113476_1440.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMAWEERFIT.
OrthoDBEOG622PMP.
ProtClustDBPRK08508.

Enzyme and pathway databases

BioCycHPYL563041:GC38-1368-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_HELPG
AccessionPrimary (citable) accession number: B5Z928
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 25, 2008
Last modified: February 19, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways