B5Z8Q2 (PYRE_HELPG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	HPG27_1202

Organism

Helicobacter pylori (strain G27) [Complete proteome] [HAMAP]

Taxonomic identifier

563041 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000138797
Regions
	Region	113 – 121	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	117	1	Orotate By similarity
	Binding site	145	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5Z8Q2 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 8579AA6E8162C826	FASTA	201	21,964
	10 20 30 40 50 60 MDIKACYQNA KALLEGHFLL SSGFHSNYYL QSAKVLEDPK LAEQLALELA KQIQEAHLNI 70 80 90 100 110 120 ECVCSPAIGG ILAGYELARA LGVRFIFTER VDNTMTLRRG FEVKKNEKIL VCEDIITTGK 130 140 150 160 170 180 SAMECAKVLE EKGAQIVAFG ALANRGICKR THSHLKAQEG ACLPSHLPLF ALEDFVFDMH 190 200 KPSSCPLCAT SVAIKPGSRG N

References

[1]

"The complete genome sequence of Helicobacter pylori strain G27." Baltrus D.A., Amieva M.R., Covacci A., Lowe T.M., Merrell D.S., Ottemann K.M., Stein M., Salama N.R., Guillemin K. J. Bacteriol. 191:447-448(2009) [PubMed: 18952803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001173 Genomic DNA. Translation: ACI27951.1.
RefSeq	YP_002266817.1. NC_011333.1.
3D structure databases
ProteinModelPortal	B5Z8Q2.
ModBase	Search...
Protein-protein interaction databases
STRING	B5Z8Q2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6964118.
GenomeReviews	Gene locus HPG27_1202 in contig CP001173_GR.
KEGG	hpg:HPG27_1202.
PATRIC	20600544. VBIHelPyl113476_1303.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404341.
OMA	FYLQSAK.
ProtClustDB	PRK00455.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR023031. Orotate_PribosylTferase. IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. PyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_HELPG

Accession

Primary (citable) accession number: B5Z8Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program