Skip Header

Contribute Send feedback
Read comments (?) or add your own

B5Z7F5 (DEF_HELPG) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide deformylase
Short name=PDF
EC=3.5.1.88
Alternative name(s):
Polypeptide deformylase
Gene names
Name:def
Ordered Locus Names:HPG27_749
OrganismHelicobacter pylori (strain G27) [Complete proteome] [HAMAP]
Taxonomic identifier563041 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length174 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity. HAMAP MF_00163

Sequence similarities

Belongs to the polypeptide deformylase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtranslation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functioniron ion binding

Inferred from electronic annotation. Source: InterPro

peptide deformylase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 174174Peptide deformylase HAMAP MF_00163
PRO_1000097315

Sites

Active site1391 By similarity
Metal binding961Iron By similarity
Metal binding1381Iron By similarity
Metal binding1421Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
B5Z7F5 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 2618800C45EFFB49

FASTA17420,069
        10         20         30         40         50         60 
MALLEIIHYP SKILRTISKE VVSFDAKLHQ QLDDMHETMI ASEGIGLAAI QVGLPLRMLI 

        70         80         90        100        110        120 
INLPREDGVQ HKEDCLEIIN PKFIETGGSM MYREGCLSVP GFYEEVERFE KVKIEYQNRF 

       130        140        150        160        170 
AEVKVLEASE LLAVAIQHEI DHLNGVLFVD KLSILKRKKF EKELKELQKK QKHK

« Hide

References

[1]"The complete genome sequence of Helicobacter pylori strain G27."
Baltrus D.A., Amieva M.R., Covacci A., Lowe T.M., Merrell D.S., Ottemann K.M., Stein M., Salama N.R., Guillemin K.
J. Bacteriol. 191:447-448(2009) [PubMed: 18952803] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: G27.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001173 Genomic DNA. Translation: ACI27504.1.
RefSeqYP_002266370.1. NC_011333.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB5Z7F5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6963639.
GenomeReviewsGene locus HPG27_749 in contig CP001173_GR.
KEGGhpg:HPG27_749.
PATRIC20599500. VBIHelPyl113476_0805.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG665227.
OMAETMIASE.
ProtClustDBPRK00150.

Family and domain databases

HAMAPMF_00163. Pep_deformylase.
[Tree]
InterProIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
Gene3DG3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KOK01462.
PANTHERPTHR10458. Fmet_deformylase. 1 hit.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
SUPFAMSSF56420. Fmet_deformylase. 1 hit.
TIGRFAMsTIGR00079. Pept_deformyl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDEF_HELPG
AccessionPrimary (citable) accession number: B5Z7F5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 25, 2008
Last modified: January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents