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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Helicobacter pylori (strain G27)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotationSAAS annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotationSAAS annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+SAAS annotationNote: Binds 1 Mg2+ ion per subunit.SAAS annotation
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311UDP-GlcNAcUniRule annotation
Metal bindingi116 – 1161MagnesiumUniRule annotation
Binding sitei148 – 1481UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei162 – 1621UDP-GlcNAcUniRule annotation
Binding sitei177 – 1771UDP-GlcNAcUniRule annotation
Metal bindingi234 – 2341MagnesiumUniRule annotation
Binding sitei234 – 2341UDP-GlcNAcUniRule annotation
Binding sitei321 – 3211Acetyl-CoA; amide nitrogenUniRule annotation
Binding sitei338 – 3381Acetyl-CoAUniRule annotation
Active sitei349 – 3491Proton acceptorUniRule annotation
Binding sitei352 – 3521Acetyl-CoAUniRule annotation
Binding sitei363 – 3631Acetyl-CoAUniRule annotation
Binding sitei391 – 3911Acetyl-CoAUniRule annotation
Binding sitei409 – 4091Acetyl-CoA; via amide nitrogenUniRule annotation
Binding sitei426 – 4261Acetyl-CoAUniRule annotation

GO - Molecular functioni

  1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB-HAMAP
  2. cell wall organization Source: UniProtKB-KW
  3. lipid A biosynthetic process Source: UniProtKB-UniPathway
  4. lipopolysaccharide biosynthetic process Source: InterPro
  5. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  6. regulation of cell shape Source: UniProtKB-KW
  7. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

AcyltransferaseUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciHPYL563041:GC38-653-MONOMER.
UniPathwayiUPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotationSAAS annotation
Gene namesi
Name:glmUUniRule annotationImported
Ordered Locus Names:HPG27_641Imported
OrganismiHelicobacter pylori (strain G27)Imported
Taxonomic identifieri563041 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000001735: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotrimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi563041.HPG27_641.

Structurei

3D structure databases

ProteinModelPortaliB5Z751.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 236236PyrophosphorylaseUniRule annotationAdd
BLAST
Regioni17 – 204UDP-GlcNAc bindingUniRule annotation
Regioni90 – 912UDP-GlcNAc bindingUniRule annotation
Regioni237 – 25721LinkerUniRule annotationAdd
BLAST
Regioni258 – 443186N-acetyltransferaseUniRule annotationAdd
BLAST
Regioni372 – 3732Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation
In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiFAHARPK.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B5Z751-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKFPKKRIL MLSVIILAAG KGTRMRSGLP KTLHTICGEP MLFYILETAF
60 70 80 90 100
SISNDVHLVL HHQQERIKEA VLERFKGVIF HTQIVEKYSG TGGAIMQEDK
110 120 130 140 150
TPIPTKHERV LILNADMPLI TKDALTPLLE SKNNAIGLLH LADPKGYGRV
160 170 180 190 200
VLENHQVKKI VEEKDANDEE KTIKSVNAGV YFFERKFLEK YLPKLNDQNA
210 220 230 240 250
QKEYYLTDLI ALGINENETI DAIFLEEECF LGVNRQTERA KAEEIMLERL
260 270 280 290 300
RKNAMDLGVV MQLPKSIYLE KGVSFKGECV LEQGVRLIGN CLIENARIKA
310 320 330 340 350
YSVIEESQII NSSVGPFAHA RPKSVICDSH VGNFVETKNA KLQGTKAGHL
360 370 380 390 400
SYLGDCEIGK NTNVGAGVIT CNYDGKKKHQ TIIGENVFIG SDSQLVAPIT
410 420 430 440
IGSNVLIGSG TTITKDIPSG SLSLSRAPQI NIENGYFKFF KKP
Length:443
Mass (Da):49,224
Last modified:November 25, 2008 - v1
Checksum:i31C009BEBD36E708
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001173 Genomic DNA. Translation: ACI27400.1.
RefSeqiYP_002266266.1. NC_011333.1.

Genome annotation databases

EnsemblBacteriaiACI27400; ACI27400; HPG27_641.
GeneIDi6963530.
KEGGihpg:HPG27_641.
PATRICi20599261. VBIHelPyl113476_0686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001173 Genomic DNA. Translation: ACI27400.1.
RefSeqiYP_002266266.1. NC_011333.1.

3D structure databases

ProteinModelPortaliB5Z751.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi563041.HPG27_641.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI27400; ACI27400; HPG27_641.
GeneIDi6963530.
KEGGihpg:HPG27_641.
PATRICi20599261. VBIHelPyl113476_0686.

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiFAHARPK.
OrthoDBiEOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00533.
UPA00973.
BioCyciHPYL563041:GC38-653-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G27Imported.

Entry informationi

Entry nameiB5Z751_HELPG
AccessioniPrimary (citable) accession number: B5Z751
Entry historyi
Integrated into UniProtKB/TrEMBL: November 25, 2008
Last sequence update: November 25, 2008
Last modified: February 4, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported, Multifunctional enzymeUniRule annotationSAAS annotation

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.