B5Z4E4 (B5Z4E4_ECO5E) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Thymidylate synthase HAMAP MF_00008
Short name=TS HAMAP MF_00008
Short name=TSase HAMAP MF_00008
EC=2.1.1.45 HAMAP MF_00008

Gene names

Name:	thyA HAMAP MF_00008
Ordered Locus Names:	ECH74115_4093

Organism

Escherichia coli O157:H7 (strain EC4115 / EHEC) [Complete proteome] [HAMAP] EMBL ACI39681.1

Taxonomic identifier

444450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008 SAAS SAAS023451
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008 SAAS SAAS023451
Subunit structure	Homodimer By similarity. HAMAP MF_00008 SAAS SAAS023451
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. HAMAP MF_00008

Ontologies

Keywords
Biological process	Nucleotide biosynthesis SAAS SAAS023451 HAMAP MF_00008
Cellular component	Cytoplasm HAMAP MF_00008
Molecular function	Methyltransferase HAMAP MF_00008 SAAS SAAS023451 EMBL ACI39681.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	146	1	By similarity HAMAP MF_00008

Sequences

	Sequence		Length	Mass (Da)
	B5Z4E4 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 0E6D88ED98D24D22	FASTA	264	30,480
	10 20 30 40 50 60 MKQYLELMQK VLDEGTQKND RTGTGTLSIF GHQMRFNLQD GFPLVTTKRC HLRSIIHELL 70 80 90 100 110 120 WFLQGDTNIA YLHENNVTIW DEWADENGDL GPVYGKQWRA WPTPDGRHID QITTVLNQLK 130 140 150 160 170 180 NDPDSRRIIV SAWNVGELDK MALAPCHAFF QFYVADGKLS CQLYQRSCDV FLGLPFNIAS 190 200 210 220 230 240 YALLVHMMAQ QCDLEVGDFV WTGGDTHLYS NHMDQTHLQL SREPRPLPKL IIKRKPESIF 250 260 DYRFEDFEIE GYDPHPGIKA PVAI

References

[1]	"Complete genome sequence of Escherichia coli O157:H7 str. EC4115." Eppinger M., Sebastian Y., Ravel J. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001164 Genomic DNA. Translation: ACI39681.1.
RefSeq	YP_002272290.1. NC_011353.1.
3D structure databases
ProteinModelPortal	B5Z4E4.
SMR	B5Z4E4. Positions 2-264.
ModBase	Search...
Protein-protein interaction databases
STRING	B5Z4E4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000032886; EBESCP00000031589; EBESCG00000031936.
GeneID	6968581.
GenomeReviews	Gene locus ECH74115_4093 in contig CP001164_GR.
KEGG	ecf:ECH74115_4093.
PATRIC	18368805. VBIEscCol74651_4030.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009308.
HOGENOM	HBG588098.
OMA	DIPRMKL.
ProtClustDB	PRK01827.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B5Z4E4_ECO5E

Accession

Primary (citable) accession number: B5Z4E4

Entry history

Integrated into UniProtKB/TrEMBL:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)