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Protein

Glycerol-3-phosphate acyltransferase

Gene

plsB

Organism
Escherichia coli O157:H7 (strain EC4115 / EHEC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.UniRule annotation

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase (plsB)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferaseUniRule annotation (EC:2.3.1.15UniRule annotation)
Short name:
GPATUniRule annotation
Gene namesi
Name:plsBUniRule annotation
Ordered Locus Names:ECH74115_5522
OrganismiEscherichia coli O157:H7 (strain EC4115 / EHEC)
Taxonomic identifieri444450 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001230761 – 807Glycerol-3-phosphate acyltransferaseAdd BLAST807

Structurei

3D structure databases

ProteinModelPortaliB5Z182
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi305 – 310HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.UniRule annotation

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218231
KOiK00631
OMAiEVIYVPC

Family and domain databases

HAMAPiMF_00393 Glyc3P_acyltrans, 1 hit
InterProiView protein in InterPro
IPR022284 GPAT/DHAPAT
IPR028354 GPAT_PlsB
IPR002123 Plipid/glycerol_acylTrfase
PANTHERiPTHR12563 PTHR12563, 1 hit
PfamiView protein in Pfam
PF01553 Acyltransferase, 1 hit
PIRSFiPIRSF500064 GPAT, 1 hit
PIRSF000437 GPAT_DHAPAT, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit
TIGRFAMsiTIGR03703 plsB, 1 hit

Sequencei

Sequence statusi: Complete.

B5Z182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGWPRIYYK LLNLPLSILV KSKSIPADPA PELGLDTSRP IMYVLPYNSK
60 70 80 90 100
ADLLTLRAQC LAHDLPDPLE PLEIDGTLLP RYVFIHGGPR VFTYYTPKEE
110 120 130 140 150
SIKLFHDYLD LHRSNPNLDV QMVPVSVMFG RAPGREKGEV NPPLRMLNGV
160 170 180 190 200
QKFFAVLWLG RDSFVRFSPS VSLRRMADEH GTDKTIAQKL ARVARMHFAR
210 220 230 240 250
QRLAAVGPRL PARQDLFNKL LASRAIAKAV EDEARSKKIS HEKAQQNAIA
260 270 280 290 300
LMEEIAANFS YEMIRLTDRI LGFTWNRLYQ GINVHNAERV RQLAHDGHEL
310 320 330 340 350
VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA GPIFRRLGAF
360 370 380 390 400
FIRRTFKGNK LYSTVFREYL GELFSRGYSV EYFVEGGRSR TGRLLDPKTG
410 420 430 440 450
TLSMTIQAML RGGTRPITLI PIYIGYEHVM EVGTYAKELR GATKEKESLP
460 470 480 490 500
QMLRGLSKLR NLGQGYVNFG EPMPLMTYLN QHVPDWRESI DPIEAVRPAW
510 520 530 540 550
LTPTVNNIAA DLMVRINNAG AANAMNLCCT ALLASRQRSL TREQLTEQLN
560 570 580 590 600
CYLDLMRNVP YSTDSTVPSA SASELIDHAL QMNKFEVEKD TIGDIIILPR
610 620 630 640 650
EQAVLMTYYR NNIAHMLVLP SLMAAIVTQH RHISRDVLME HVNVLYPMLK
660 670 680 690 700
AELFLRWDRD ELPDVIDALA NEMQRQGLIT LQDDELHINP VHSRTLQLLA
710 720 730 740 750
AGARETLQRY AITFWLLSAN PSINRGTLEK ESRTVAQRLS VLHGINAPEF
760 770 780 790 800
FDKAVFSSLV LTLRDEGYIS DSGDAEPAET MKVYQLLAEL ITSDVRLTIE

SATQGEG
Length:807
Mass (Da):91,409
Last modified:November 25, 2008 - v1
Checksum:i26FC7F929639F593
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001164 Genomic DNA Translation: ACI38153.1
RefSeqiWP_001301502.1, NC_011353.1

Genome annotation databases

EnsemblBacteriaiACI38153; ACI38153; ECH74115_5522
KEGGiecf:ECH74115_5522

Similar proteinsi

Entry informationi

Entry nameiPLSB_ECO5E
AccessioniPrimary (citable) accession number: B5Z182
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: March 28, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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