B5YVL1 (SYGB_ECO5E) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Ordered Locus Names:	ECH74115_4934

Organism

Escherichia coli O157:H7 (strain EC4115 / EHEC) [Complete proteome] [HAMAP]

Taxonomic identifier

444450 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli O157:H7

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	Glycine--tRNA ligase beta subunit HAMAP-Rule MF_00255		PRO_1000101277

Sequences

	Sequence		Length	Mass (Da)
	B5YVL1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 3F9B939FEA3168CD	FASTA	689	76,813
	10 20 30 40 50 60 MSEKTFLVEI GTEELPPKAL RSLAESFAAN FTAELDNAGL AHGTVQWFAA PRRLALKVAN 70 80 90 100 110 120 LAEAQPDREI EKRGPAIAQA FDAEGKPSKA AEGWARGCGI TVDQAERLTT DKGEWLLYRA 130 140 150 160 170 180 HVKGESTEAL LPNMVATSLA KLPIPKLMRW GASDVHFVRP VHTVTLLLGD KVIPATILGI 190 200 210 220 230 240 QSDRVIRGHR FMGEPEFTID NADQYPEILR ERGKVIADYE ERKAKIKADA EEAARKIGGN 250 260 270 280 290 300 ADLSESLLEE VASLVEWPVV LTAKFEEKFL AVPSEALVYT MKGDQKYFPV YANDGKLLPN 310 320 330 340 350 360 FIFVANIESK DPQQIISGNE KVVRPRLADA EFFFNTDRKK RLEDNLPRLQ TVLFQQQLGT 370 380 390 400 410 420 LRDKTDRIQA LAGWIAEQIG ADVNHATRAG LLSKCDLMTN MVFEFTDTQG VMGMHYARHD 430 440 450 460 470 480 GEAEDVAVAL NEQYQPRFAG DDLPSNPVAC ALAIADKMDT LAGIFGIGQH PKGDKDPFAL 490 500 510 520 530 540 RRAALGVLRI IVEKNLNLDL QTLTEEAVRL YGDKLTNANV VDDVIDFMLG RFRAWYQDEG 550 560 570 580 590 600 YTVDTIQAVL ARRPTRPADF DARMKAVSHF RTLEAAAALA AANKRVSNIL AKSDEVLSDR 610 620 630 640 650 660 VNASTLKEPE EIKLAMQVVV LRDKLEPYFA EGRYQDALVE LAELREPVDA FFDKVMVMVD 670 680 DKELRINRLT MLEKLRELFL RVADISLLQ

References

[1]	"Complete genome sequence of Escherichia coli O157:H7 str. EC4115." Eppinger M., Sebastian Y., Ravel J. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EC4115 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001164 Genomic DNA. Translation: ACI38550.1.
RefSeq	YP_002273044.1. NC_011353.1.
3D structure databases
ProteinModelPortal	B5YVL1.
ModBase	Search...
Protein-protein interaction databases
STRING	444450.ECH74115_4934.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI38550; ACI38550; ECH74115_4934.
GeneID	6970455.
KEGG	ecf:ECH74115_4934.
PATRIC	18370423. VBIEscCol74651_4825.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0751.
HOGENOM	HOG000264302.
KO	K01879.
OMA	GMHYARL.
ProtClustDB	PRK01233.
Enzyme and pathway databases
BioCyc	ECOL444450:GHOB-4323-MONOMER.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta.
InterPro	IPR008909. DALR_anticod-bd. IPR015944. Gly-tRNA-synth_bsu. IPR006194. Gly-tRNA-synth_heterodimer. [Graphical view]
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
SMART	SM00836. DALR_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00211. glyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_ECO5E

Accession

Primary (citable) accession number: B5YVL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program