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Protein

Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB

Gene

rutB

Organism
Escherichia coli O157:H7 (strain EC4115 / EHEC)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2.UniRule annotation

Catalytic activityi

(Z)-3-ureidoacrylate peracid + H2O = (Z)-3-peroxyaminoacrylate + CO2 + NH3.UniRule annotation
(Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + CO2 + NH3.UniRule annotation
(Z)-2-methyl-ureidoacrylate peracid + H2O = (Z)-2-methyl-peroxyaminoacrylate + CO2 + NH3.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei167 – 1671NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciECOL444450:GHOB-1244-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBUniRule annotation (EC:3.5.1.110UniRule annotation)
Alternative name(s):
Ureidoacrylate amidohydrolaseUniRule annotation
Gene namesi
Name:rutBUniRule annotation
Ordered Locus Names:ECH74115_1248
OrganismiEscherichia coli O157:H7 (strain EC4115 / EHEC)
Taxonomic identifieri444450 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 231231Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBPRO_0000402672Add
BLAST

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB5YU52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isochorismatase family. RutB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000078669.
KOiK09020.
OMAiLAKGGWD.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB. 1 hit.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.

Sequencei

Sequence statusi: Complete.

B5YU52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTTLTARPE AITFDPQQSA LIVVDMQNAY ATPGGYLDLA GFDVSTTRPV
60 70 80 90 100
IANIQTAVTA ARAAGMLIIW FQNGWDEQYV EAGGPGSPNF HKSNALKTMR
110 120 130 140 150
KQPQLQGKLL AKGSWDYQLV DELVPQPGDI VLPKPRYSGF FNTPLDSILR
160 170 180 190 200
SRGIRHLVFT SIATNVCVES TLRDGFFLEY FGVVLEDATH QAGPEFVQKA
210 220 230
ALFNIETFFG WVSDVETFCD ALSPTSFARI A
Length:231
Mass (Da):25,418
Last modified:November 25, 2008 - v1
Checksum:iFEDB6113E2C59577
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001164 Genomic DNA. Translation: ACI39728.1.

Genome annotation databases

EnsemblBacteriaiACI39728; ACI39728; ECH74115_1248.
KEGGiecf:ECH74115_1248.
PATRICi18363290. VBIEscCol74651_1330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001164 Genomic DNA. Translation: ACI39728.1.

3D structure databases

ProteinModelPortaliB5YU52.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI39728; ACI39728; ECH74115_1248.
KEGGiecf:ECH74115_1248.
PATRICi18363290. VBIEscCol74651_1330.

Phylogenomic databases

HOGENOMiHOG000078669.
KOiK09020.
OMAiLAKGGWD.

Enzyme and pathway databases

BioCyciECOL444450:GHOB-1244-MONOMER.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB. 1 hit.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUTB_ECO5E
AccessioniPrimary (citable) accession number: B5YU52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 25, 2008
Last modified: September 7, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.