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Protein

FMN reductase (NADH) RutF

Gene

rutF

Organism
Escherichia coli O157:H7 (strain EC4115 / EHEC)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway.UniRule annotation

Catalytic activityi

FMNH2 + NAD+ = FMN + NADH.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NAD

Enzyme and pathway databases

BioCyciECOL444450:GHOB-1240-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
FMN reductase (NADH) RutFUniRule annotation (EC:1.5.1.42UniRule annotation)
Alternative name(s):
FMN reductaseUniRule annotation
NADH-flavin reductase RutFUniRule annotation
NADH:flavin oxidoreductaseUniRule annotation
Gene namesi
Name:rutFUniRule annotation
Ordered Locus Names:ECH74115_1244
OrganismiEscherichia coli O157:H7 (strain EC4115 / EHEC)
Taxonomic identifieri444450 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 164164FMN reductase (NADH) RutFPRO_0000403013Add
BLAST

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB5YU49.
SMRiB5YU49. Positions 4-159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the non-flavoprotein flavin reductase family. RutF subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000115783.
KOiK09024.
OMAiWFDRGYH.
OrthoDBiEOG6T1WSH.

Family and domain databases

Gene3Di2.30.110.10. 1 hit.
HAMAPiMF_00833. RutF.
InterProiIPR002563. Flavin_Rdtase-like_dom.
IPR019917. RutF.
IPR012349. Split_barrel_FMN-bd.
[Graphical view]
PfamiPF01613. Flavin_Reduct. 1 hit.
[Graphical view]
SMARTiSM00903. Flavin_Reduct. 1 hit.
[Graphical view]
SUPFAMiSSF50475. SSF50475. 1 hit.
TIGRFAMsiTIGR03615. RutF. 1 hit.

Sequencei

Sequence statusi: Complete.

B5YU49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIVDQQTFR DAMSCMGAAV NIITTDGPAG RAGFTASAVC SVTDTPPTLL
60 70 80 90 100
VCLNRGASVW PVFNENRTLC VNTLSAGQEP LSNLFGGKTP MEHRFAAARW
110 120 130 140 150
QNGVTGCPQL EEALVSFDCR ISQVVSVGTH DILFCAIEAI HRHATPYGLV
160
WFDRSYHALM RPAC
Length:164
Mass (Da):17,760
Last modified:November 25, 2008 - v1
Checksum:i78778237EF228380
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001164 Genomic DNA. Translation: ACI36661.1.
RefSeqiWP_001028088.1. NC_011353.1.

Genome annotation databases

EnsemblBacteriaiACI36661; ACI36661; ECH74115_1244.
KEGGiecf:ECH74115_1244.
PATRICi18363282. VBIEscCol74651_1326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001164 Genomic DNA. Translation: ACI36661.1.
RefSeqiWP_001028088.1. NC_011353.1.

3D structure databases

ProteinModelPortaliB5YU49.
SMRiB5YU49. Positions 4-159.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI36661; ACI36661; ECH74115_1244.
KEGGiecf:ECH74115_1244.
PATRICi18363282. VBIEscCol74651_1326.

Phylogenomic databases

HOGENOMiHOG000115783.
KOiK09024.
OMAiWFDRGYH.
OrthoDBiEOG6T1WSH.

Enzyme and pathway databases

BioCyciECOL444450:GHOB-1240-MONOMER.

Family and domain databases

Gene3Di2.30.110.10. 1 hit.
HAMAPiMF_00833. RutF.
InterProiIPR002563. Flavin_Rdtase-like_dom.
IPR019917. RutF.
IPR012349. Split_barrel_FMN-bd.
[Graphical view]
PfamiPF01613. Flavin_Reduct. 1 hit.
[Graphical view]
SMARTiSM00903. Flavin_Reduct. 1 hit.
[Graphical view]
SUPFAMiSSF50475. SSF50475. 1 hit.
TIGRFAMsiTIGR03615. RutF. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EC4115 / EHEC.

Entry informationi

Entry nameiRUTF_ECO5E
AccessioniPrimary (citable) accession number: B5YU49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 25, 2008
Last modified: November 11, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.