Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B5YTX2

- RTCA_ECO5E

UniProt

B5YTX2 - RTCA_ECO5E

Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Escherichia coli O157:H7 (strain EC4115 / EHEC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (25 Nov 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

    Catalytic activityi

    ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei103 – 1031ATPUniRule annotation
    Active sitei308 – 3081Tele-AMP-histidine intermediateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi283 – 2875ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL444450:GHOB-4709-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
    Short name:
    RNA cyclaseUniRule annotation
    Short name:
    RNA-3'-phosphate cyclaseUniRule annotation
    Gene namesi
    Name:rtcAUniRule annotation
    Ordered Locus Names:ECH74115_4728
    OrganismiEscherichia coli O157:H7 (strain EC4115 / EHEC)
    Taxonomic identifieri444450 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001737: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 342342RNA 3'-terminal phosphate cyclasePRO_1000099346Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi444450.ECH74115_4728.

    Structurei

    3D structure databases

    ProteinModelPortaliB5YTX2.
    SMRiB5YTX2. Positions 4-337.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0430.
    HOGENOMiHOG000015264.
    KOiK01974.
    OMAiERTRTGH.
    OrthoDBiEOG6RNQDX.

    Family and domain databases

    Gene3Di3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPiMF_00200. RTC.
    InterProiIPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view]
    PANTHERiPTHR11096. PTHR11096. 1 hit.
    PfamiPF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view]
    SUPFAMiSSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 2 hits.
    TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEiPS01287. RTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B5YTX2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRMIALDGA QGEGGGQIMR SALSLSMITG QPFTITGIRA GRAKPGLLRQ    50
    HLTAVKAATE ICGATVEGAE LGSQRLVFRP GTVRGGDYRF AIGSAGSCTL 100
    VLQTVLPALW FADGPSRVEV SGGTDNPSAP PADFIRRVLE PLLAKIGIHQ 150
    QTTLLRHGFY PAGGGVVATE VSPVASFNTL QLGERGNIVQ MRGEVLLAGV 200
    PRHVAEREIA TLAASFSLHE QNIHNLPRDQ GPGNTVSLEV ESENITERFF 250
    VVGEKRVSAE VVAAQLVKEV KRYLASPAAV GEYLADQLVL PMALAGAGQF 300
    TVAHPSCHLL TNIAVVERFL PVRFTLAETD GVTRVMITKL TD 342
    Length:342
    Mass (Da):36,332
    Last modified:November 25, 2008 - v1
    Checksum:i783FE7FAD7160846
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001164 Genomic DNA. Translation: ACI38089.1.
    RefSeqiYP_002272857.1. NC_011353.1.

    Genome annotation databases

    EnsemblBacteriaiACI38089; ACI38089; ECH74115_4728.
    GeneIDi6971165.
    KEGGiecf:ECH74115_4728.
    PATRICi18370025. VBIEscCol74651_4627.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001164 Genomic DNA. Translation: ACI38089.1 .
    RefSeqi YP_002272857.1. NC_011353.1.

    3D structure databases

    ProteinModelPortali B5YTX2.
    SMRi B5YTX2. Positions 4-337.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 444450.ECH74115_4728.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACI38089 ; ACI38089 ; ECH74115_4728 .
    GeneIDi 6971165.
    KEGGi ecf:ECH74115_4728.
    PATRICi 18370025. VBIEscCol74651_4627.

    Phylogenomic databases

    eggNOGi COG0430.
    HOGENOMi HOG000015264.
    KOi K01974.
    OMAi ERTRTGH.
    OrthoDBi EOG6RNQDX.

    Enzyme and pathway databases

    BioCyci ECOL444450:GHOB-4709-MONOMER.

    Family and domain databases

    Gene3Di 3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPi MF_00200. RTC.
    InterProi IPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view ]
    PANTHERi PTHR11096. PTHR11096. 1 hit.
    Pfami PF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 2 hits.
    TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEi PS01287. RTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: EC4115 / EHEC.

    Entry informationi

    Entry nameiRTCA_ECO5E
    AccessioniPrimary (citable) accession number: B5YTX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3