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B5YT98 (RLMI_ECO5E) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase I
EC=2.1.1.191
Alternative name(s):
23S rRNA m5C1962 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RlmI
Gene names
Name:rlmI
Ordered Locus Names:ECH74115_1132
OrganismEscherichia coli O157:H7 (strain EC4115 / EHEC) [Complete proteome] [HAMAP]
Taxonomic identifier444450 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA By similarity. HAMAP-Rule MF_01857

Catalytic activity

S-adenosyl-L-methionine + cytosine(1962) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1962) in 23S rRNA. HAMAP-Rule MF_01857

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01857.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmI family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

rRNA (cytosine) methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396Ribosomal RNA large subunit methyltransferase I HAMAP-Rule MF_01857
PRO_0000366229

Regions

Domain2 – 8180PUA

Sequences

Sequence LengthMass (Da)Tools
B5YT98 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 413E90026B7C2B7D

FASTA39644,357
        10         20         30         40         50         60 
MSVRLVLAKG REKSLLRRHP WVFSGAVARM EGKASLGETI DIVDHQGKWL ARGAYSPASQ 

        70         80         90        100        110        120 
IRARVWTFDP SESIDIAFFS RRLQQAQKWR DWLAQKDGLD SYRLIAGESD GLPGITIDRF 

       130        140        150        160        170        180 
GNFLVLQLLS AGAEYQRAAL ISALQTLYPE CAIYDRSDVA VRKKEGMELT QGLVTGELPP 

       190        200        210        220        230        240 
ALLPIEEHGM KLLVDIQHGH KTGYYLDQRD SRLATRRYVE NKRVLNCFSY TGGFAVSALM 

       250        260        270        280        290        300 
GGCSQVVSVD TSQEALDIAR QNVELNKLDL SKAEFVRDDV FKLLRTYRDR GEKFDVIVMD 

       310        320        330        340        350        360 
PPKFVENKSQ LMGACRGYKD INMLAIQLLN EGGILLTFSC SGLMTSDLFQ KIIADAAIDA 

       370        380        390 
GRDVQFIEQF RQAADHPVIA TYPEGLYLKG FACRVM

« Hide

References

[1]"Complete genome sequence of Escherichia coli O157:H7 str. EC4115."
Eppinger M., Sebastian Y., Ravel J.
Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: EC4115 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001164 Genomic DNA. Translation: ACI39814.1.
RefSeqYP_002269636.1. NC_011353.1.

3D structure databases

ProteinModelPortalB5YT98.
SMRB5YT98. Positions 2-396.
ModBaseSearch...

Protein-protein interaction databases

STRING444450.ECH74115_1132.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACI39814; ACI39814; ECH74115_1132.
GeneID6969020.
KEGGecf:ECH74115_1132.
PATRIC18363065. VBIEscCol74651_1221.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1092.
HOGENOMHOG000266009.
KOK06969.
OMACSGLMTT.
ProtClustDBPRK15128.

Enzyme and pathway databases

BioCycECOL444450:GHOB-5677-MONOMER.

Family and domain databases

HAMAPMF_01857. 23SrRNA_methyltr_I.
InterProIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR023542. rRNA_lsu_MeTfrase_I.
IPR019614. SAM-dep_methyl-trfase.
[Graphical view]
PfamPF10672. Methyltrans_SAM. 1 hit.
[Graphical view]
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF88697. PUA-like. 1 hit.
PROSITEPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRLMI_ECO5E
AccessionPrimary (citable) accession number: B5YT98
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: November 25, 2008
Last modified: May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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