Reviewed, UniProtKB/Swiss-Prot B5YT65 (PNCB_ECO5E)

Names and origin

Protein names

Recommended name:
    Nicotinate phosphoribosyltransferase
      Short name=NAPRTase
    EC=2.4.2.11

Gene names

Name:	pncB
Ordered Locus Names:	ECH74115_1092

Organism

Escherichia coli O157:H7 (strain EC4115 / EHEC) [Complete proteome] [HAMAP]

Taxonomic identifier

444450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00570
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. HAMAP MF_00570
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAPRTase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP nicotinate nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nicotinate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Nicotinate phosphoribosyltransferase HAMAP MF_00570		PRO_1000129466

Sequences

	Sequence		Length	Mass (Da)
	B5YT65-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 6D75FF3488FCD4BA	FASTA	400	45,969
	10 20 30 40 50 60 MTQFASPVLH SLLDTDAYKL HMQQAVFHHY YDVHVAAEFR CRGDDLLGIY ADAIREQVQA 70 80 90 100 110 120 MQHLRLQDDE YQWLSALPFF KADYLNWLRE FRFNPEQVTV SNDNGKLDIR LSGPWREVIL 130 140 150 160 170 180 WEVPLLAVIS EMVHRYRSPQ ADVAQALDTL ESKLVDFSAL TAGLDMSRFH LMDFGTRRRF 190 200 210 220 230 240 SREVQETIVK RLQQESWFVG TSNYDLARRL SLTPMGTQAH EWFQAHQQIS PDLANSQRAA 250 260 270 280 290 300 LAAWLEEYPD QLGIALTDCI TMDAFLRDFG VEFASRYQGL RHDSGDPVEW GEKAIAHYEK 310 320 330 340 350 360 LEIDPQSKTL VFSDNLDLRK AVELYRHFSS RVQLSFGIGT RLTCDIPQVK PLNIVIKLVE 370 380 390 400 CNGKPVAKLS DSPGKTICHD KAFVRALRKA FDLPHIKKAS

References

[1]	"Complete genome sequence of Escherichia coli O157:H7 str. EC4115." Eppinger M., Sebastian Y., Ravel J. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001164 Genomic DNA. Translation: ACI39222.1.
RefSeq	YP_002269603.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6967970.
GenomeReviews	Gene locus ECH74115_1092 in contig CP001164_GR.
KEGG	ecf:ECH74115_1092.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ALTDTFG.
Family and domain databases
HAMAP	MF_00570. [Tree]
InterPro	IPR006406. Nic_PRibTrfase. IPR015977. Nic_PRibTrfase-like. IPR007229. Nic_PRibTrfase-rel. [Graphical view]
PANTHER	PTHR11098:SF1. NAPRTase. 1 hit.
Pfam	PF04095. NAPRTase. 1 hit. [Graphical view]
PIRSF	PIRSF000484. NAPRT. 1 hit.
TIGRFAMs	TIGR01514. NAPRTase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNCB_ECO5E

Accession

Primary (citable) accession number: B5YT65

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)