B5YT20 (LFTR_ECO5E) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucyl/phenylalanyl-tRNA--protein transferase
EC=2.3.2.6

Alternative name(s):
L/F-transferase
Leucyltransferase
Phenyalanyltransferase

Gene names

Name:	aat
Ordered Locus Names:	ECH74115_1047

Organism

Escherichia coli O157:H7 (strain EC4115 / EHEC) [Complete proteome] [HAMAP]

Taxonomic identifier

444450 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli O157:H7

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine By similarity. HAMAP-Rule MF_00688
Catalytic activity	L-leucyl-tRNA + protein = tRNA + L-leucyl-protein. HAMAP-Rule MF_00688 L-phenylalanyl-tRNA + protein = tRNA + L-phenylalanyl-protein. HAMAP-Rule MF_00688
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L/F-transferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	leucyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	Leucyl/phenylalanyl-tRNA--protein transferase HAMAP-Rule MF_00688		PRO_1000131919

Sequences

	Sequence		Length	Mass (Da)
	B5YT20 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 8C725890DE8A156F	FASTA	234	26,618
	10 20 30 40 50 60 MRLVQLSRHS IAFPSPEGAL REPNGLLALG GDLSPARLLM AYQRGIFPWF SPGDPILWWS 70 80 90 100 110 120 PDPRAVLWPE SLHISRSMKR FHKRSPYRVT MNYAFGQVIE GCASDREEGT WITRGVVEAY 130 140 150 160 170 180 HRLHELGHAH SIEVWREDEL VGGMYGVAQG TLFCGESMFS RMENASKTAL LVFCEEFIGH 190 200 210 220 230 GGKLIDCQVL NDHTASLGAC EIPRRNYLNY LNQMRLGRLP NNFWVPRCLF SPQE

References

[1]	"Complete genome sequence of Escherichia coli O157:H7 str. EC4115." Eppinger M., Sebastian Y., Ravel J. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: EC4115 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001164 Genomic DNA. Translation: ACI38322.1.
RefSeq	YP_002269558.1. NC_011353.1.
3D structure databases
ProteinModelPortal	B5YT20.
SMR	B5YT20. Positions 2-233.
ModBase	Search...
Protein-protein interaction databases
STRING	444450.ECH74115_1047.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACI38322; ACI38322; ECH74115_1047.
GeneID	6968276.
KEGG	ecf:ECH74115_1047.
PATRIC	18362885. VBIEscCol74651_1131.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2360.
HOGENOM	HOG000102325.
KO	K00684.
OMA	MFYGESM.
ProtClustDB	PRK00301.
Enzyme and pathway databases
BioCyc	ECOL444450:GHOB-4077-MONOMER.
Family and domain databases
HAMAP	MF_00688. Leu_Phe_trans.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR004616. Leu/Phe-tRNA_Trfase. [Graphical view]
Pfam	PF03588. Leu_Phe_trans. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR00667. aat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LFTR_ECO5E

Accession

Primary (citable) accession number: B5YT20

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program