Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein deglycase HchA

Gene

hchA

Organism
Escherichia coli O157:H7 (strain EC4115 / EHEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Plays an important role in protecting cells from carbonyl stress.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861ZincUniRule annotation
Metal bindingi91 – 911ZincUniRule annotation
Metal bindingi123 – 1231ZincUniRule annotation
Active sitei185 – 1851NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciECOL444450:GHOB-2740-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein deglycase HchAUniRule annotation (EC:3.1.2.-UniRule annotation, EC:3.5.1.-UniRule annotation)
Gene namesi
Name:hchAUniRule annotation
Ordered Locus Names:ECH74115_2746
OrganismiEscherichia coli O157:H7 (strain EC4115 / EHEC)
Taxonomic identifieri444450 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Protein deglycase HchAPRO_1000136175Add
BLAST

Expressioni

Inductioni

By heat shock.UniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB5YSJ3.
SMRiB5YSJ3. Positions 5-283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C56 family. HchA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000221774.
KOiK05523.
OMAiPMYHLDK.
OrthoDBiEOG65J50B.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01046. Prot_deglyc_HchA.
InterProiIPR029062. Class_I_gatase-like.
IPR017283. HchA.
[Graphical view]
PIRSFiPIRSF037798. Chaperone_HchA. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

B5YSJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVQTSKNPQ VDIAEDNAFF PSEYSLSQYT SPVSDLDGVD YPKPYRGKHK
60 70 80 90 100
ILVIAADERY LPTDNGKLFS TGNHPIETLL PLYHLHAAGF EFEVATISGL
110 120 130 140 150
MTKFEYWAMP QKDEKVMPFF EQHKSLFRNP KKLADVVASL NADSEYAAIF
160 170 180 190 200
VPGGHGALIG LPESQDVAAA LQWAIKNDRF VISLCHGPAA FLALRHGDNP
210 220 230 240 250
LNGYSICAFP DAADKQTPEI GYMPGHLTWY FGEELKKMGM NIINDDITGR
260 270 280
VHKDRKLLTG DSPFAANALG KLAAQEMLAA YAG
Length:283
Mass (Da):31,181
Last modified:November 25, 2008 - v1
Checksum:iE36046A5F8325F98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001164 Genomic DNA. Translation: ACI36666.1.
RefSeqiWP_000218228.1. NC_011353.1.

Genome annotation databases

EnsemblBacteriaiACI36666; ACI36666; ECH74115_2746.
KEGGiecf:ECH74115_2746.
PATRICi18366204. VBIEscCol74651_2770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001164 Genomic DNA. Translation: ACI36666.1.
RefSeqiWP_000218228.1. NC_011353.1.

3D structure databases

ProteinModelPortaliB5YSJ3.
SMRiB5YSJ3. Positions 5-283.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI36666; ACI36666; ECH74115_2746.
KEGGiecf:ECH74115_2746.
PATRICi18366204. VBIEscCol74651_2770.

Phylogenomic databases

HOGENOMiHOG000221774.
KOiK05523.
OMAiPMYHLDK.
OrthoDBiEOG65J50B.

Enzyme and pathway databases

BioCyciECOL444450:GHOB-2740-MONOMER.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01046. Prot_deglyc_HchA.
InterProiIPR029062. Class_I_gatase-like.
IPR017283. HchA.
[Graphical view]
PIRSFiPIRSF037798. Chaperone_HchA. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: EC4115 / EHEC.

Entry informationi

Entry nameiHCHA_ECO5E
AccessioniPrimary (citable) accession number: B5YSJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 25, 2008
Last modified: June 8, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.