Reviewed, UniProtKB/Swiss-Prot B5YQD6 (SPEA_ECO5E)

Names and origin

Protein names

Recommended name:
    Biosynthetic arginine decarboxylase
      Short name=ADC
    EC=4.1.1.19

Gene names

Name:	speA
Ordered Locus Names:	ECH74115_4241

Organism

Escherichia coli O157:H7 (strain EC4115 / EHEC) [Complete proteome] [HAMAP]

Taxonomic identifier

444450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	632 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the biosynthesis of agmatine from arginine By similarity. HAMAP MF_01417
Catalytic activity	L-arginine = agmatine + CO2. HAMAP MF_01417
Cofactor	Magnesium By similarity. HAMAP MF_01417 Pyridoxal phosphate By similarity. HAMAP MF_01417
Pathway	Amine and polyamine biosynthesis; agmatine biosynthesis; agmatine from L-arginine: step 1/1. HAMAP MF_01417
Sequence similarities	Belongs to the Orn/Lys/Arg decarboxylase class-II family. SpeA subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Putrescine biosynthesis Spermidine biosynthesis
Ligand	Magnesium Metal-binding Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process Inferred from electronic annotation. Source: InterPro putrescine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	arginine decarboxylase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 632	632	Biosynthetic arginine decarboxylase HAMAP MF_01417		PRO_1000145592
Regions
	Region	281 – 291	11	Substrate-binding Potential
Amino acid modifications
	Modified residue	101	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B5YQD6-1 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: EE36CC59AF9D2569	FASTA	632	71,181
	10 20 30 40 50 60 MSSQEASKML RTYNIAWWGN NYYDVNELGH ISVCPDPDVP EARVDLAQLV KTREAQGQRL 70 80 90 100 110 120 PALFCFPQIL QHRLRSINAA FKRARESYGY NGDYFLVYPI KVNQHRRVIE SLIHSGEPLG 130 140 150 160 170 180 LEAGSKAELM AVLAHAGMTR SVIVCNGYKD REYIRLALIG EKMGHKVYLV IEKMSEIAIV 190 200 210 220 230 240 LDEAERLNVV PRLGVRARLA SQGSGKWQSS GGEKSKFGLA ATQVLQLVET LREAGRLDSL 250 260 270 280 290 300 QLLHFHLGSQ MANIRDIATG VRESARFYVE LHKLGVNIQC FDVGGGLGVD YEGTRSQSDC 310 320 330 340 350 360 SVNYGLNEYA NNIIWAIGDA CEENGLPHPT VITESGRAVT AHHTVLVSNI IGVERNEYTV 370 380 390 400 410 420 PTAPAEDAPR ALQSMWETWQ EMHEPGTRRS LREWLHDSQM DLHDIHIGYS SGTFSLQERA 430 440 450 460 470 480 WAEQLYLSMC HEVQKQLDPQ NRAHRPIIDE LQERMADKMY VNFSLFQSMP DAWGIDQLFP 490 500 510 520 530 540 VLPLEGLDQV PERRAVLLDI TCDSDGAIDH YIDGDGIATT MPMPEYDPEN PPMLGFFMVG 550 560 570 580 590 600 AYQEILGNMH NLFGDTEAVD VFVFPDGSVE VELSDEGDTV ADMLQYVQLD PKTLLTQFRD 610 620 630 QVKKTDLDAE LQQQFLEEFE AGLYGYTYLE DE

References

[1]	"Complete genome sequence of Escherichia coli O157:H7 str. EC4115." Eppinger M., Sebastian Y., Ravel J. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001164 Genomic DNA. Translation: ACI37609.1.
RefSeq	YP_002272418.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6968274.
GenomeReviews	Gene locus ECH74115_4241 in contig CP001164_GR.
KEGG	ecf:ECH74115_4241.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG321436.
OMA	LICNGYK.
Family and domain databases
HAMAP	MF_01417. SpeA. [Tree]
InterPro	IPR002985. Arg_decrbxlase. IPR000183. De-COase2. [Graphical view]
PANTHER	PTHR11482:SF3. Arg_decrbxlase. 1 hit.
Pfam	PF02784. Orn_Arg_deC_N. 1 hit. PF00278. Orn_DAP_Arg_deC. 1 hit. [Graphical view]
PIRSF	PIRSF001336. Arg_decrbxlase. 1 hit.
PRINTS	PR01180. ARGDCRBXLASE. PR01179. ODADCRBXLASE.
TIGRFAMs	TIGR01273. speA. 1 hit.
PROSITE	PS00878. ODR_DC_2_1. 1 hit. PS00879. ODR_DC_2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEA_ECO5E

Accession

Primary (citable) accession number: B5YQD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 25, 2008
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)